Accuracy of invasive and noninvasive methods of Helicobacter pylori infection diagnosis in Saudi children.

Background/Aim: Helicobacter pylori (H. pylori) infection is one of the most common chronic infections in the world. The prevalence of H. pylori is high in Saudi Arabia, but there are no studies in children on the accuracy of invasive and noninvasive methods to diagnose H. pylori infection. The aim...

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Publicado en:Saudi Journal of Gastroenterology Vol. 25; no. 2; pp. 126 - 132
Autor principal: Hasosah, Mohammed
Formato: research tables/charts Journal Article
Publicado: Wolters Kluwer India Pvt Ltd Mar/Apr2019
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: Saudi Journal of Gastroenterology
      issn: 13193767
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    pubinfo:
      dt: Mar/Apr2019
      vid: 25
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      pid: 16919
      pub: Wolters Kluwer India Pvt Ltd
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        135627077
        135627077
        135627077
        10.4103/sjg.SJG_288_18
        135627077
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      tig:
        atl: Accuracy of invasive and noninvasive methods of Helicobacter pylori infection diagnosis in Saudi children.
      aug:
        au: Hasosah, Mohammed
        affil: Department of Pediatric Gastroenterology, King Abdulaziz Medical City, King Saud bin Abdulaziz University for Health Sciences, Jeddah
      sug:
        subj:
          Invasive Procedures Methods
          Noninvasive Procedures Methods
          Helicobacter Infections Diagnosis
          Pediatric Care
          Sensitivity and Specificity
          Human
          Validity
          Prospective Studies
          Cross Sectional Studies
          Saudi Arabia
          Hospitals, Pediatric
          Endoscopy, Digestive System
          Tissue Culture Techniques
          Prevalence
          Helicobacter Infections Epidemiology
          Confidence Intervals
          Child
          Urease Analysis
          Histological Techniques Methods
          Biopsy Methods
          Serologic Tests
          Antigens, Bacterial Analysis
          Feces Analysis
          Child: 6-12 years
      ab: Background/Aim: Helicobacter pylori (H. pylori) infection is one of the most common chronic infections in the world. The prevalence of H. pylori is high in Saudi Arabia, but there are no studies in children on the accuracy of invasive and noninvasive methods to diagnose H. pylori infection. The aim of this study was to evaluate the accuracy of six methods for diagnosis of H. pylori infection; four invasive [rapid urease test (RUT), histology, antral nodularity (AD), and biopsy culture (BC)] and two noninvasive methods [serologic test and stool antigen test (SAT)]. Patients and Methods: A prospective cross-sectional study was performed among symptomatic children in National Guard hospitals who underwent esophagogastroduodenoscopy from 2010 to 2013. The gold standard diagnosis of H. pylori was positive tissue culture. If the culture was unsuccessful or not done, concordant-positive results for histology and RUT were considered to indicate a positive H. pylori. The variables analyzed as diagnostic methods included RUT, BC, histology, AD, serologic test, and SAT. Results: A total of 303 children were included in the study. The overall prevalence of H. pylori infection was 49.8%. Most diagnostic tests showed high specificity and moderate-to-low sensitivity when compared to the gold standard test. Sensitivity of AD, SAT, and RUT to detect H. pylori were 62% (95% CI: 0.51–0.74), 69% (95% CI: 0.58–0.79), and 87% (95% CI: 0.79–0.95), respectively (P value 0.040, 0.0023, and <0.0001, respectively). RUT showed the lowest specificity, 65% (95% CI: 0.58–0.71) in contrast to BC and histology which showed moderate-to-high specificities of 88% (95% CI: 0.82–0.95) and 89% (95% CI: 0.82–0.95), respectively (P <0.0001). Conclusion: RUT is a valuable diagnostic method for identifying H. pylori with the highest sensitivity compared to AD and SAT. All diagnostic tests showed moderate-to-high specificities but BC and histology showed the highest specificity.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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