Frequency-induced morphology alterations in microconfined biological cells.
Low-intensity therapeutic ultrasound has demonstrated an impetus in bone signaling and tissue healing for decades now. Though this technology is clinically well proven, still there are breaches in studies to understand the fundamental principle of how osteoblast tissue regenerates physiologically at...
| Published in: | Medical & Biological Engineering & Computing Vol. 57; no. 4; pp. 819 - 836 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Published: |
Springer Nature
Apr2019
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=135753226&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 135753226 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01400118 PO0 jtl: Medical & Biological Engineering & Computing issn: 01400118 maglogo: N pubinfo: dt: Apr2019 vid: 57 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 135753226 135753226 NLM30415434 10.1007/s11517-018-1908-y NLM30415434 135753226 ppf: 819 ppct: 17 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Frequency-induced morphology alterations in microconfined biological cells. aug: au: Banerjee, Hritwick Roy, Bibhas Chaudhury, Kaustav Srinivasan, Babji Chakraborty, Suman Ren, Hongliang affil: Department of Electrical Engineering, Indian Institute of Technology Gandhinagar, Village Palaj Simkheda, 382355, Gandhinagar, Gujarat, India sug: subj: Cells Cell Physiology Ultrasonics Cell Line, Tumor Muscle Proteins Metabolism Cell Size Time Factors Kinematics Cytoplasm Metabolism Clinical Assessment Tools ab: Low-intensity therapeutic ultrasound has demonstrated an impetus in bone signaling and tissue healing for decades now. Though this technology is clinically well proven, still there are breaches in studies to understand the fundamental principle of how osteoblast tissue regenerates physiologically at the cellular level with ultrasound interaction as a form of acoustic wave stimuli. Through this article, we illustrate an analysis for cytomechanical changes of cell membrane periphery as a basic first physical principle for facilitating late downstream biochemical pathways. With the help of in situ single-cell direct analysis in a microfluidic confinement, we demonstrate that alteration of low-intensity pulse ultrasound (LIPUS) frequency would physically perturb cell membrane and establish inherent cell oscillation. We experimentally demonstrate here that, at LIPUS resonance near 1.7 MHz (during 1-3 MHz alteration), cell membrane area would expand to 6.85 ± 0.7% during ultrasound exposure while it contracts 44.68 ± 0.8% in post actuation. Conversely, cell cross-sectional area change (%) from its previous morphology during and after switching off LIPUS was reversibly different before and after resonance. For instance, at 1.5 MHz, LIPUS exposure produced 1.44 ± 0.5% expansion while in contrast 2 MHz instigates 1.6 ± 0.3% contraction. We conclude that alteration of LIPUS frequency from 1-3 MHz keeping other ultrasound parameters like exposure time, pulse repetition frequency (PRF), etc., constant, if applied to a microconfined biological single living cell, would perturb physical structure reversibly based on the system resonance during and post exposure ultrasound pulsing. We envision, in the near future, our results would constitute the foundation of mechanistic effects of low-intensity therapeutic ultrasound and its allied potential in medical applications. Graphical Abstract Frequency Dependent Characterization of Area Strain in Cell Membrane by Microfluidic Based Single Cell Analysis. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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