Identification and Determination of Antibiotic Resistance Pattern of Stenotrophomonas maltophilia Isolated form Medical Devices and Clinical Specimens in Jahrom,s Hospitals by Phenotype and Molecular Methods.
Introduction: Stenotrophomonas maltophilia is an important cause of nosocomial infections. The bacteria is a nonfermentative gram-negative bacilli. S. maltophilia is a newly emerging pathogen of growing significance that has been more frequently isolated in nosocomial specimens, hospital waters and...
| Publicado en: | Pars Journal of Medical Sciences Vol. 14; no. 2; pp. 42 - 50 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Journal of Jahrom University of Medical Sciences
Summer2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=135856631&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 135856631 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 27170284 N98H jtl: Pars Journal of Medical Sciences issn: 27170284 maglogo: N pubinfo: dt: Summer2016 vid: 14 iid: 2 pid: 70438 pub: Journal of Jahrom University of Medical Sciences place: , <Blank> artinfo: ui: 135856631 135856631 135856631 10.29252/jmj.14.2.43 135856631 ppf: 42 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Identification and Determination of Antibiotic Resistance Pattern of Stenotrophomonas maltophilia Isolated form Medical Devices and Clinical Specimens in Jahrom,s Hospitals by Phenotype and Molecular Methods. aug: au: Nikpour, Aboutaleb Shabani, Manoochehr Kazemi, Akbar Mohandesi, Maryam Ershdpour, Roya Yazdi, Hadi Rezaei affil: Dept of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran sug: subj: Gram-Negative Aerobic Bacteria Analysis Drug Resistance, Microbial Specimen Handling Iran Equipment Contamination Iran Phenotype Molecular Diagnostic Techniques Microbial Culture and Sensitivity Tests Random Sample Staining and Labeling Polymerase Chain Reaction Ticarcillin Chloramphenicol Ceftazidime Ofloxacin Aztreonam Trimethoprim-Sulfamethoxazole Combination Imipenem Cross Infection Human ab: Introduction: Stenotrophomonas maltophilia is an important cause of nosocomial infections. The bacteria is a nonfermentative gram-negative bacilli. S. maltophilia is a newly emerging pathogen of growing significance that has been more frequently isolated in nosocomial specimens, hospital waters and disinfection solutions. S. maltophilia is inherently resistant to many antimicrobial drugs that cause a significant challenge in treatment of infections. Most strains of this bacteria are resistant to many antibiotics, including: carbapenems, macrolides, cephalosporins, fluoroquinolones, aminoglycosides. Materials & Methods: In this study, 170 samples from medical equipment and 250 clinical specimens were collected randomly from patients who were referred to hospitals in Jahrom city. Samples were transported to the laboratory in sterile containers. Then were cultured on phenotypic differential medium. After isolation of the bacteria, Gram staining was performed to detect gram-negative bacilli. After the identification of gramnegative bacilli, biochemical and differential tests were performed to identify the genus and species of S. maltophilia. Molecular diagnostic tests (PCR) was performed for confirmation of bacteria. Results: Of the 250 clinical specimen, 14 samples (5.6%) and of the 170 samples collected from medical equipment, only 1 sample (0.6%) were positive. Positive samples were studied in terms of sensitivity to several antibiotics. Antibiotics were included: Ticarcillin (71.4%), Ceftazidime (64.3%), Levofloxacin (78.57%), Trimethoprim-sulfamethoxazole (7.14%), Chloramphenicol (28.57%), Aztreonam (64.3%), Imipenem (71.4%). Conclusion: According to prevalence, high levels of antibiotic resistance and increase of multiple drug resistance (MDR) in stenotrophomonas maltophilia, identification and determination of antibiotic resistance is essential and recommended for treatment of opportunistic infection caused by this bacteria. It also recommended that further studies be conducted periodically in hospital on colonization of this bacteria for prevention and control. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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