Cloning tagD gene from helicobacter pylori in PFLAG-CMV-3 eukaryotic vector to generate a DNA vaccine.
Introduction: Helicobacter pylori is strongly associated with gastritis, stomach cancer, gastric lymphoma and peptic ulcer in human. Thiol peroxidase is encoded by tagD gene and plays a significant role in colonizing H. pylori in the stomach. The product of tagD gene stimulates the immune system in...
| Publicado en: | Pars Journal of Medical Sciences Vol. 14; no. 4; pp. 43 - 51 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Journal of Jahrom University of Medical Sciences
Winter2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=135856644&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 135856644 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 27170284 N98H jtl: Pars Journal of Medical Sciences issn: 27170284 maglogo: N pubinfo: dt: Winter2016 vid: 14 iid: 4 pid: 70438 pub: Journal of Jahrom University of Medical Sciences place: , <Blank> artinfo: ui: 135856644 135856644 135856644 10.29252/jmj.14.4.43 135856644 ppf: 43 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Cloning tagD gene from helicobacter pylori in PFLAG-CMV-3 eukaryotic vector to generate a DNA vaccine. aug: au: Safarpour, Maryam Kazemi, Zahra Doosti, Elham Doosti, Abbas affil: Dept of Biology, Faculty of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran sug: subj: Genetic Techniques Helicobacter Pylori Gene Expression DNA Vaccines Animal Studies Experimental Studies Sequence Analysis Helicobacter Infections Prevention and Control Genes Physiology ab: Introduction: Helicobacter pylori is strongly associated with gastritis, stomach cancer, gastric lymphoma and peptic ulcer in human. Thiol peroxidase is encoded by tagD gene and plays a significant role in colonizing H. pylori in the stomach. The product of tagD gene stimulates the immune system in the host. This study aimed to isolate and clone tagD gene in the eukaryotic expression vector PFLAG-CMV-3 as a DNA vaccine candidate. Materials and methods: In this experimental research, tagD gene (537 bp) was amplified by PCR. The PCR products were cloned using cloning commercial kits (Thermo Fisher Co., COUNTRY) in pTZ vector. This gene was subcloned in the eukaryotic expression vector (PFLAG-CMV-3 vector), then transferred into CHO cells by electroporation method and was expressed. Results: The results indicate that amplification and cloning of tagD gene was successful, and the pTZ-tagD vector was formed. PFLAG-CMV-3 vector construction was confirmed by digestion and gene sequencing. The 19 kDa band was observed by gene expression analysis on SDS-PAGE. Conclusions: tagD gene in the PFLAG-CMV-3-tagD recombinant vector has the ability to produce specific protein in CHO cells. Therefore, this gene construct is useful to evaluate the immunogenicity as a DNA vaccine against H. pylori infection in animal models. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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