Chitosan-based composite bilayer scaffold as an in vitro osteochondral defect regeneration model.
Prolonged osteochondral tissue damage can result in osteoarthritis and decreased quality of life. Multiphasic scaffolds, where different layers model different microenvironments, are a promising treatment approach, yet stable joining between layers during fabrication remains challenging. Here, a bil...
| Publicado en: | Biomedical Microdevices Vol. 21; no. 2 |
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| Autores principales: | , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jun2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=136256474&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 136256474 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Jun2019 vid: 21 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 136256474 10.1007/s10544-019-0373-1 136256474 ppct: 1 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Chitosan-based composite bilayer scaffold as an in vitro osteochondral defect regeneration model. aug: au: Erickson, Ariane E. Sun, Jialu Lan Levengood, Sheeny K. Swanson, Shawn Chang, Fei-Chien Tsao, Ching T. Zhang, Miqin affil: Department of Materials Science & Engineering, University of Washington, 98195, Seattle, WA, USA sug: ab: Prolonged osteochondral tissue damage can result in osteoarthritis and decreased quality of life. Multiphasic scaffolds, where different layers model different microenvironments, are a promising treatment approach, yet stable joining between layers during fabrication remains challenging. Here, a bilayer scaffold for osteochondral tissue regeneration was fabricated using thermally-induced phase separation (TIPS). Two distinct polymer solutions were layered before TIPS, and the resulting porous, bilayer scaffold was characterized by seamless interfacial integration and a mechanical stiffness gradient reflecting the native osteochondral microenvironment. Chitosan is a critical component of both scaffold layers to facilitate cell attachment and the formation of polyelectrolyte complexes with other biologically relevant natural polymers. The articular cartilage region was optimized for hyaluronic acid content and stiffness, while the subchondral bone region was defined by higher stiffness and osteoconductive hydroxyapatite content. Following co-culture with chondrocyte-like (SW-1353 or mesenchymal stem cells) and osteoblast-like cells (MG63), cell proliferation and migration to the interface along with increased gene expression associated with relevant markers of osteogenesis and chondrogenesis indicates the potential of this bilayer scaffold for osteochondral tissue regeneration. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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