The effect of warming specimens of rapid urease test on its diagnostic accuracy.
Introduction: Helicobacter pylori (H. pylori) is a prevalent cause of dyspepsia, peptic ulcer and gastric cancer in developing countries. Among various diagnostic methods, rapid urease test (RUT) is the ideal test for its diagnosis in patients undergoing endoscopy. Studies have identified the factor...
| Publicado en: | Revista Latinoamericana de Hipertensión Vol. 14; no. 1; pp. 15 - 20 |
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| Autores principales: | , , , , |
| Formato: | Artículo |
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Revista Latinoamericana de Hipertension
2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=136456785&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 136456785 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 18564550 FICN jtl: Revista Latinoamericana de Hipertensión issn: 18564550 maglogo: N pubinfo: dt: 2019 vid: 14 iid: 1 pid: 81901 pub: Revista Latinoamericana de Hipertension artinfo: ui: 136456785 ppf: 15 ppct: 5 formats: fmt: @attributes: type: P size: 233KB tig: atl: The effect of warming specimens of rapid urease test on its diagnostic accuracy. aug: au: Nasiri, Jafar Allahdin, Arshya Imani, Reza Kheiri, Soleiman Khoshdel, Abolfazl affil: Gastroenterologist, Assistant Professor, Department of Internal Medicine, Faculty of Medicine, Shaharekord University of Medical Sciences, Shahrekord, Iran Assistant of Pathology, Department of Pathology, Faculty of Medicine, Jundishapour Medical Sciences University, Ahwaz, Iran Associate Professor, Department of Infectious Diseases, Shahrekord University of Medical Sciences, Shahrekord, Iran Associate Professor, Department of Epidemiology and Biostatistics, Faculty of Health, Shahrekord University of Medical Sciences, Shahrekord, Iran Associate Professor, Department of Pediatrics, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran su: Urease Clarithromycin Diagnosis Helicobacter pylori infections Peptic ulcer Helicobacter pylori sug: subj: Urease Clarithromycin Diagnosis Helicobacter pylori infections Peptic ulcer Helicobacter pylori keyword: Diagnostic Tests, Routine Endoscopy, Gastrointestinal Endoscopia gastrointestinal Pruebas de diagnóstico, de rutina ab: Introduction: Helicobacter pylori (H. pylori) is a prevalent cause of dyspepsia, peptic ulcer and gastric cancer in developing countries. Among various diagnostic methods, rapid urease test (RUT) is the ideal test for its diagnosis in patients undergoing endoscopy. Studies have identified the factors causing false negative and positive results. One of these factors is proposed to be the temperature of keeping specimens, but the results have been controversial. We aimed to compare the diagnostic accuracy of RUT specimens warmed at 37oC or kept at room temperature (22-25oC) in different time intervals until 24 hours. Methods: 100 patients with dyspepsia who were indicated for endoscopic examination were selected based on convenience sampling method from patients referring to Endoscopy center of Hajar Hospital during August-September 2006. After recording the demographic and medical history of patients, three biopsy specimens were taken from one portion of the antrum; the first two samples were placed in home-made RUT solution and the third biopsy was placed in formalin solution; one tube was placed in incubator with 37oC and the other at room temperature (22-25oC). Positivity of RUT test at different times was compared to histopathological examination. Results: Infection with Helicobacter pylori was confirmed by histological examination in 66% of patients. After 24 hours, sensitivity of incubated RUT was 71% at 37oC, and 68% at room temperature (P=0.85). Specificity at 37oC was 59% and at room temperature was 76% (P=0.19). Median time to positive tests was 2 hours at 37oC and 3 hours at room temperature, while RUT became positive faster at 37oC (P=0.553). Conclusion: The non-significant difference between groups revealed that warming could not improve the diagnostic accuracy of RUT test, thus, the standard RUT method is recommended. Introducción: Helicobacter pylori (H. Pylori) es una causa frecuente de dispepsia, úlcera péptica y cáncer gástrico en países en desarrollo. Entre los diversos métodos de diagnóstico, la prueba rápida de ureasa (RUT) es la prueba ideal para su diagnóstico en pacientes sometidos a endoscopia. Los estudios han identificado los factores que causan resultados falsos negativos y positivos. Se propone que uno de estos factores es la temperatura para conservar las muestras, pero los resultados han sido controvertidos. El objetivo fue comparar la precisión diagnóstica de las muestras RUT calentadas a 37°C o mantenidas a temperatura ambiente (22-25°C) en diferentes intervalos de tiempo hasta 24 horas. Métodos: se seleccionaron 100 pacientes con dispepsia que estaban indicados para un examen endoscópico según el método de muestreo de conveniencia de los pacientes que se referían al centro de Endoscopia del Hospital Hajar entre agosto y septiembre de 2006. Después de registrar la historia demográfica y médica de los pacientes, se tomaron tres muestras de biopsia. Una porción del antro; las dos primeras muestras se colocaron en una solución de RUT hecha en casa y la tercera biopsia se colocó en una solución de formalina; un tubo se colocó en una incubadora con 37°C y el otro a temperatura ambiente (22-25°C). La positividad de la prueba de RUT en diferentes momentos se comparó con el examen histopatológico. Resultados: la infección con Helicobacter pylori se confirmó mediante examen histológico en el 66% de los pacientes. Después de 24 horas, la sensibilidad del RUT incubado fue del 71% a 37°C y del 68% a temperatura ambiente (P=0,85). La especificidad a 37°C fue del 59% y la temperatura ambiente fue del 76% (P=0.19). El tiempo medio para las pruebas positivas fue de 2 horas a 37°C y 3 horas a temperatura ambiente, mientras que el RUT se convirtió en positivo más rápido a 37°C (P=0.553). Conclusión: la diferencia no significativa entre los grupos reveló que el calentamiento no podría mejorar la precisión diagnóstica de la prueba RUT, por lo que se recomienda el método RUT estándar. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista Latinoamericana de Hipertensión is the property of Revista Latinoamericana de Hipertension and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista Latinoamericana de Hipertensión holder: Revista Latinoamericana de Hipertension dt: @attributes: year: 2019 holdings: @attributes: islocal: N |
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