A new immunohistochemical method to evaluate the development of vestibular compensation after unilateral labyrinthectomy in rats.
Background: Unilateral labyrinthectomy (UL) causes the disappearance of ipsilateral medial vestibular nuclear (ipsi-MVe) activity and induces spontaneous nystagmus (SN), which disappears during the initial process of vestibular compensation (VC). Ipsi-MVe-activity restores in the late process of VC....
| Publicado en: | Acta Oto-Laryngologica Vol. 139; no. 6; pp. 505 - 511 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Jun2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=136461170&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 136461170 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00016489 B6D jtl: Acta Oto-Laryngologica issn: 00016489 maglogo: Y pubinfo: dt: Jun2019 vid: 139 iid: 6 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 136461170 136461170 136461170 10.1080/00016489.2019.1599140 136461170 ppf: 505 ppct: 6 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: A new immunohistochemical method to evaluate the development of vestibular compensation after unilateral labyrinthectomy in rats. aug: au: Matsuda, Kazunori Kitahara, Tadashi Ito, Taeko Fukushima, Munehisa Fukuda, Junya Sato, Go Kitamura, Yoshiaki Abe, Koji Uno, Atsuhiko Tomita, Koichi Sakata-Haga, Hiromi Fukui, Yoshihiro Takeda, Noriaki affil: Department of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan sug: subj: Immunohistochemistry Methods Vestibular Diseases Risk Factors Ear Surgery Adverse Effects Vestibular Diseases Diagnosis Postoperative Complications Histamine Antagonists Pharmacodynamics Vestibule, Labyrinth Drug Effects Animal Studies Rats Receptors, Cell Surface Antagonists and Inhibitors Cerebellum Neurons ab: Background: Unilateral labyrinthectomy (UL) causes the disappearance of ipsilateral medial vestibular nuclear (ipsi-MVe) activity and induces spontaneous nystagmus (SN), which disappears during the initial process of vestibular compensation (VC). Ipsi-MVe-activity restores in the late process of VC. Objective: We evaluated the late process of VC after UL in rats and examined the effects of thioperamide (H3 antagonist) on VC. Materials and methods: MK801 (NMDA antagonist)-induced Fos-like immunoreactive (-LIR) neurons in contra-MVe, which had been suppressed by NMDA-mediated cerebellar inhibition in UL rats was used as an index. Results: The number of MK801-induced Fos-LIR neurons in contra-MVe gradually decreased to the same level as that of sham-operated rats 14 days after UL. Thioperamide moved the disappearance of the MK801-induced Fos-LIR neurons 2 days earlier. The number of MK801-induced Fos-LIR neurons in thioperamide-treated rats was significantly decreased, compared with that of vehicle rats on days 7 and 12 after UL. But, thioperamide did not influence the decline of SN frequency in UL rats. Conclusion: These findings suggested that the number of MK801-induced Fos-LIR neurons in contra-MVe was decreased in concordance with the restoration of ipsi-MVe-activity during the late process of VC after UL and that thioperamide accelerated the late, but not the initial process of VC. 背景：单侧迷路切除术（UL）导致同侧内侧前庭核（ipsi-MVe）活动消失并诱发自发性眼球震颤（SN）, 后者在前庭补偿（VC）初期消失。 Ipsi-MVe活动在VC后期恢复。 目的：评价大鼠UL后VC的晚期过程, 并检测硫哌酰胺（H3拮抗剂）对VC的影响。 材料和方法：MK801（NMDA拮抗剂）诱导的抗MVe中的Fos样免疫反应性（-LIR）神经元被用作指标；它已经被UL大鼠的NMDA介导的小脑抑制所抑制。 结果：抗MVe中MK801诱导的Fos-LIR神经元数量在UL后14天逐渐下降至与假手术大鼠相同的水平。硫丙使得MK801诱导的Fos-LIR神经元的消失提前了两天。在UL后第7天和第12天, 与载体大鼠相比, 硫丙处理的大鼠中MK801诱导的Fos-LIR神经元的数量显著减少。但是, 硫丙并未影响UL大鼠SN频率的下降。 结论：这些研究结果表明, 在UL之后VC的后期过程中, MV801诱导的Fos-LIR神经元在抗MVe中的数量随着ipsi-MVe活性的恢复而减少, 而且硫丙加速了VC的晚期过程, 而不是它的初始过程。 pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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