Ferumoxytol Can Be Used for Quantitative Magnetic Particle Imaging of Transplanted Stem Cells.
Purpose: To evaluate, if clinically translatable ferumoxytol nanoparticles can be used for in vivo detection and quantification of stem cell transplants with magnetic particle imaging (MPI).Procedures: Mesenchymal stem cells (MSCs) were labeled with ferumoxytol or ferucarbotran and underwent MPI, ma...
| Publicado en: | Molecular Imaging & Biology Vol. 21; no. 3; pp. 465 - 473 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Jun2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=136504778&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 136504778 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Jun2019 vid: 21 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 136504778 136504778 NLM30194566 136504778 10.1007/s11307-018-1276-x NLM30194566 136504778 ppf: 465 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Ferumoxytol Can Be Used for Quantitative Magnetic Particle Imaging of Transplanted Stem Cells. aug: au: Nejadnik, Hossein Pandit, Prachi Lenkov, Olga Lahiji, Arian Pourmehdi Yerneni, Ketan Daldrup-Link, Heike E. affil: Pediatric Molecular Imaging Program in the Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University School of Medicine, 725 Welch Rd, Rm 1665, 94305-5654, Stanford, CA, USA sug: subj: Molecular Imaging Stem Cells Transplantation Ferric Compounds Physical Sciences Mice Magnetic Resonance Imaging Skull Dextrans Female Spectrophotometry Animal Studies Skull Pathology Validation Studies Comparative Studies Evaluation Research Multicenter Studies Female ab: Purpose: To evaluate, if clinically translatable ferumoxytol nanoparticles can be used for in vivo detection and quantification of stem cell transplants with magnetic particle imaging (MPI).Procedures: Mesenchymal stem cells (MSCs) were labeled with ferumoxytol or ferucarbotran and underwent MPI, magnetic resonance imaging (MRI), Prussian blue staining, and inductively coupled plasma (ICP) spectrometry. Unlabeled, ferumoxytol, and ferucarbotran-labeled MSCs were implanted in calvarial defects of eight mice and underwent MPI, MRI, and histopathology. The iron concentration calculated according to the MPI signal intensity and T2 relaxation times of the three different groups were compared using an analysis of variance (ANOVA) with Bonferroni correction, and a p < 0.05.Results: Compared to unlabeled controls, ferumoxytol- and ferucarbotran-labeled MSC showed significantly increased iron content, MPI signal and MRI signal. The ferumoxytol MPI signal was approximately 4× weaker compared to ferucarbotran at equimolar concentrations (p = 0.0003) and approximately 1.5× weaker for labeled cells when using optimized labeling protocols (p = 0.002). In vivo, the MPI signal of ferumoxytol-labeled MSC decreased significantly between day 1 and day 14 (p = 0.0124). This was confirmed by histopathology where we observed a decrease in Prussian blue stain of MSCs at the transplant site. The MRI signal of the same transplants did not change significantly during this observation period (p = 0.93).Conclusion: Ferumoxytol nanoparticles can be used for in vivo detection of stem cell transplants with MPI and provide quantitative information not attainable with MRI. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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