Optical Redox Imaging Detects the Effects of DEK Oncogene Knockdown on the Redox State of MDA-MB-231 Breast Cancer Cells.
Purpose: Optical redox imaging (ORI), based on collecting the endogenous fluorescence of reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavoproteins (Fp) containing a redox cofactor flavin adenine dinucleotide (FAD), provides sensitive indicators of cellular metabolism and redox stat...
| Publicado en: | Molecular Imaging & Biology Vol. 21; no. 3; pp. 410 - 417 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Jun2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=136504781&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 136504781 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Jun2019 vid: 21 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 136504781 136504781 NLM30758703 136504781 10.1007/s11307-019-01321-w NLM30758703 136504781 ppf: 410 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Optical Redox Imaging Detects the Effects of DEK Oncogene Knockdown on the Redox State of MDA-MB-231 Breast Cancer Cells. aug: au: Wen, Yu Xu, He N. Privette Vinnedge, Lisa Feng, Min Li, Lin Z. affil: Rutgers Cancer Institute of New Jersey, 08903, New Brunswick, NJ, USA sug: subj: Proteins Oncogenes Breast Neoplasms Metabolism Diagnostic Imaging Breast Neoplasms Genetic Techniques Breast Neoplasms Pathology Neoplasm Invasiveness Cell Line, Tumor Oxidation-Reduction Female Clinical Assessment Tools Funding Source Female ab: Purpose: Optical redox imaging (ORI), based on collecting the endogenous fluorescence of reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavoproteins (Fp) containing a redox cofactor flavin adenine dinucleotide (FAD), provides sensitive indicators of cellular metabolism and redox status. ORI indices (such as NADH, FAD, and their ratio) have been under investigation as potential progression/prognosis biomarkers for cancer. Higher FAD redox ratio (i.e., FAD/(FAD + NADH)) has been associated with higher invasive/metastatic potential in tumor xenografts and cultured cells. This study is to examine whether ORI indices can respond to the modulation of oncogene DEK activities that change cancer cell invasive/metastatic potential.Procedures: Using lentiviral shRNA, DEK gene expression was efficiently knocked down in MDA-MB-231 breast cancer cells (DEKsh). These DEKsh cells, along with scrambled shRNA-transduced control cells (NTsh), were imaged with a fluorescence microscope. In vitro invasive potential of the DEKsh cells and NTsh cells was also measured in parallel using the transwell assay.Results: FAD and FAD redox ratios in polyclonal cells with DEKsh were significantly lower than that in NTsh control cells. Consistently, the DEKsh cells demonstrated decreased invasive potential than their non-knockdown counterparts NTsh cells.Conclusions: This study provides direct evidence that oncogene activities could mediate ORI-detected cellular redox state. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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