Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis.
In this study, comparative experiments were carried out to investigate the positional selectivity of Candida sp. 99–125 lipase in preparing 1,3-diolein by using medium engineering strategy. The results indicated that the diolein yield was markedly enhanced from 56.5% to 86.7% with increasing logP v...
| Publicado en: | BioMed Research International pp. 1 - 8 |
|---|---|
| Autores principales: | , , , , , , , |
| Formato: | equations & formulas research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
5/20/2019
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=136533444&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 136533444 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 5/20/2019 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 136533444 136533444 136533444 10.1155/2019/4318631 136533444 ppf: 1 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis. aug: au: Bi, Yanhong Duan, Zhangqun Zhang, Wenjing Xu, Lei Wang, Zhaoyu Zhao, Xiaojuan Zhao, Xiangjie Yang, Jiali affil: School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China sug: subj: Candida Microbiologic Phenomena Lipase Metabolism Chemistry Glycerides Metabolism Tissue Engineering Fungi Experimental Studies Descriptive Statistics Solvents Glycerin Hydroxides Enzymes Industry ab: In this study, comparative experiments were carried out to investigate the positional selectivity of Candida sp. 99–125 lipase in preparing 1,3-diolein by using medium engineering strategy. The results indicated that the diolein yield was markedly enhanced from 56.5% to 86.7% with increasing logP values of the solvents, while the selectivity of the examined lipase for the sn-1 over the sn-2 hydroxyl of glycerol was decreased, thus leading to a reduced 1,3-diolein to 1,2-diolein ratio. To evaluate the possibility of industrial enzymatic production of 1,3-diolein, larger-scale experiments were assessed. After being used repeatedly for eight batches, the diolein content reached 95.1%, while the 1,3-diolein to 1,2-diolein ratio was 7:1 following purification. Results of the kg level experiments significantly demonstrated the practicability of the enzymatic process and the efficiency of the purification strategy for the product. pubtype: Academic Journal doctype: equations & formulas research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|