Dried blood spot testing for estimation of renal function and analysis of metformin and sitagliptin concentrations in diabetic patients: a cross-sectional study.

Purpose: Dried blot spot (DBS) analysis of drugs or clinical parameters offers many advantages. We investigated the feasibility of using DBS for analysis of anti-diabetic drugs concomitantly with the estimated creatinine clearance (Clcrea). Methods: The cross-sectional study involved physicians in a...

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Publicado en:European Journal of Clinical Pharmacology Vol. 75; no. 6; pp. 809 - 817
Autores principales: Scherf-Clavel, Maike, Albert, Edwin, Zieher, Stephan, Valotis, Anagnostis, Hickethier, Thomas, Högger, Petra
Formato: research tables/charts Journal Article
Publicado: Springer Nature Jun2019
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jun2019
      vid: 75
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      pub: Springer Nature
      place: New York, New York
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        136694085
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        136694085
        10.1007/s00228-019-02637-w
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        atl: Dried blood spot testing for estimation of renal function and analysis of metformin and sitagliptin concentrations in diabetic patients: a cross-sectional study.
      aug:
        au:
          Scherf-Clavel, Maike
          Albert, Edwin
          Zieher, Stephan
          Valotis, Anagnostis
          Hickethier, Thomas
          Högger, Petra
        affil: Institut für Pharmazie und Lebensmittelchemie, Universität Würzburg, Am Hubland, 97074, Würzburg, Germany
      sug:
        subj:
          Kidney Function Tests
          Blood Chemical Analysis Methods
          Metformin Blood
          Sitagliptin Blood
          Creatine Blood
          Diabetic Patients
          Human
          Adult
          Middle Age
          Aged
          Aged, 80 and Over
          Clinical Trials
          Body Mass Index
          Polypharmacy
          Diuretics
          Antiinflammatory Agents, Non-Steroidal
          Renin-Angiotensin System
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Aged: 65+ years
          Aged, 80 & over
      ab: Purpose: Dried blot spot (DBS) analysis of drugs or clinical parameters offers many advantages. We investigated the feasibility of using DBS for analysis of anti-diabetic drugs concomitantly with the estimated creatinine clearance (Clcrea). Methods: The cross-sectional study involved physicians in an enabling analysis with 70 diabetic patients and community pharmacists in a field investigation with 84 participants. All 154 DBS samples were analyzed for creatinine, metformin, and sitagliptin. Results: The diabetic patients revealed of a wide range of age (32–88 years), BMI values (19.8–54.7 kg/m2), and extent of polypharmacotherapy (1–21 drugs). A correlation factor to convert capillary blood creatinine from DBS into plasma concentrations was determined. Patients' Clcrea ranged from 21.6–155.9 mL/min. The results indicated statistically significant correlations (p < 0.05) between the use of two or three particular drug classes (diuretics, NSAIDs, renin-angiotensin system blockers) and a decreased renal function. DBS concentrations of metformin ranged between 0.23–4.99 μg/mL. The estimated elimination half-life (t ½) of metformin was 11.9 h in patients with a ClCrea higher than 60 mL/min and 18.5 h for diabetics with lower ClCrea. Sitagliptin capillary blood concentrations ranged between 11.12–995.6 ng/mL. Calculated t ½ of sitagliptin were 8.4 h and 13.0 h in patients with a ClCrea above and below 60 mL/min, respectively. Conclusions: DBS allow for the analysis of concentrations of predominantly renally eliminated drugs and community pharmacists can provide a valuable contribution to DBS sampling.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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