Isolation of Helicobacter pylori from human faeces.
Helicobacter pylori is arguably the commonest chronic infection in man. However, its route of transmission is unknown. We have isolated viable H pylori from the faeces of an infected individual from The Gambia. The organism was cultured on selective media after concentration of faecal bacteria by ce...
| Publicado en: | Lancet Vol. 340; no. 8829; pp. 1194 - 1196 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lancet
11/14/1992
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=136950334&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 136950334 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01406736 LAN jtl: Lancet issn: 01406736 maglogo: N pubinfo: dt: 11/14/1992 vid: 340 iid: 8829 pid: 1297 pub: Lancet place: Philadelphia, Pennsylvania artinfo: ui: 136950334 136950334 NLM1359263 136950334 10.1016/0140-6736(92)92894-l NLM1359263 136950334 ppf: 1194 ppct: 2 formats: tig: atl: Isolation of Helicobacter pylori from human faeces. aug: au: Thomas, J E Gibson, G R Darboe, M K Dale, A Weaver, L T affil: MRC Dunn Nutrition Centre, Cambridge, UK sug: subj: Helicobacter Pylori Feces Microbiology Helicobacter Pylori Immunology Antigens, Bacterial Analysis Electrophoresis Human Infant Male Adult Helicobacter Pylori Classification Child, Preschool Gambia Validation Studies Comparative Studies Evaluation Research Multicenter Studies Scales Infant: 1-23 months Adult: 19-44 years Child, Preschool: 2-5 years Male ab: Helicobacter pylori is arguably the commonest chronic infection in man. However, its route of transmission is unknown. We have isolated viable H pylori from the faeces of an infected individual from The Gambia. The organism was cultured on selective media after concentration of faecal bacteria by centrifugation in a buffer equilibrated with a microaerophilic gas mixture. Growth characteristics, microscopic appearances, and enzyme activities were the same as those of a typical gastric isolate of H pylori. Protein preparations derived from the new isolate and the typical strain were antigenically similar, and had very similar electrophoretic profiles (including two major protein bands of 62 and 26 kDa, corresponding to the urease enzyme subunits). With the same technique, organisms with the colony morphology, growth requirements, enzyme activities, and microscopic appearances of H pylori were isolated from the faeces of 9 of 23 randomly selected children aged 3-27 months from a Gambian village with a high prevalence of H pylori infection in early life. Faecal-oral transmission is probably important in the spread of infection in such communities. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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