Expression of homeobox gene transcripts in trophoblastic cells.
Objective: This study was conducted to examine the dynamics of homeobox gene expression in the differentiation of trophoblasts as a key to the understanding of the regulatory mechanisms that are involved in placental development.Study Design: Expression of homeobox genes was examined in primary trop...
| Publicado en: | American Journal of Obstetrics & Gynecology Vol. 187; no. 1; pp. 24 - 33 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Jul2002
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=137061025&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 137061025 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00029378 AOG jtl: American Journal of Obstetrics & Gynecology issn: 00029378 maglogo: N pubinfo: dt: Jul2002 vid: 187 iid: 1 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 137061025 NLM12114884 137061025 10.1067/mob.2002.122850 NLM12114884 137061025 ppf: 24 ppct: 9 formats: tig: atl: Expression of homeobox gene transcripts in trophoblastic cells. aug: au: Zhang, Yue Mei Xu, Bo Rote, Neal Peterson, Linda Amesse, Lawrence S affil: Department of Obstetrics/Gynecology, Wright State University School of Medicine and College of Science and Mathematics, Dayton, Ohio 45409-2793, USA sug: subj: Proteins Blastocyst Genes Pregnancy Placenta Cells, Cultured Choriocarcinoma Gene Expression Human Tumor Cells, Cultured Uterine Neoplasms Immunohistochemistry Reverse Transcriptase Polymerase Chain Reaction Female Validation Studies Comparative Studies Evaluation Research Multicenter Studies Female ab: Objective: This study was conducted to examine the dynamics of homeobox gene expression in the differentiation of trophoblasts as a key to the understanding of the regulatory mechanisms that are involved in placental development.Study Design: Expression of homeobox genes was examined in primary trophoblastic cells and in the BeWo choriocarcinoma model cell lines by molecular and immunocytochemistry techniques.Results: We demonstrated the expression of 3 homeobox genes (HOX B6, HOX C6, and HOX A11) in primary trophoblastic cells. BeWo cells showed an expression pattern similar to that of the primary cell lines. In both primary trophoblasts and BeWo cells, the HOX A11 gene, but not the HOX B6 or HOX C6 genes, were found to down-regulate with differentiation from single- to multinucleate giant cells.Conclusion: This study demonstrates a novel expression pattern for HOX A11 gene in trophoblastic differentiation and suggests that the down-regulation of HOX A11 may be necessary for the differentiation of cytotrophoblasts into syncytiotrophoblasts. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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