Immune cell score in pancreatic cancer-comparison of hotspot and whole-section techniques.
An immune cell score (ICS) was introduced for predicting survival in pancreatic ductal adenocarcinoma (PDAC). Few studies have compared different methods of evaluating immune infiltrate. This study compared ICSs determined in whole sections or tissue microarray-like hotspots for predicting survival...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 474; no. 6; pp. 691 - 700 |
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| Autores principales: | , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jun2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=137077679&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 137077679 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: Jun2019 vid: 474 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 137077679 137077679 NLM30843106 10.1007/s00428-019-02549-1 NLM30843106 137077679 ppf: 691 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Immune cell score in pancreatic cancer-comparison of hotspot and whole-section techniques. aug: au: Tahkola, Kyösti Leppänen, Joni Ahtiainen, Maarit Väyrynen, Juha Haapasaari, Kirsi-Maria Karttunen, Tuomo Kellokumpu, Ilmo Helminen, Olli Böhm, Jan affil: Department of Surgery, Central Finland Central Hospital, Jyvaskyla, Finland sug: subj: Adenocarcinoma Pathology Carcinoma, Ductal Pathology Pancreatic Neoplasms Pathology Middle Age Prognosis Survival Retrospective Design Adult Aged Aged, 80 and Over Carcinoma, Ductal Immunology Pancreatic Neoplasms Immunology Male Female Adenocarcinoma Immunology Pancreatic Neoplasms Diagnosis Middle Aged: 45-64 years Adult: 19-44 years Aged: 65+ years Aged, 80 & over Male Female ab: An immune cell score (ICS) was introduced for predicting survival in pancreatic ductal adenocarcinoma (PDAC). Few studies have compared different methods of evaluating immune infiltrate. This study compared ICSs determined in whole sections or tissue microarray-like hotspots for predicting survival after PDAC surgery. We included in 79 consecutive patients from a single geographical area that underwent surgery for PDAC (R0/R1, stages I-III). We performed digital image analyses to evaluate CD3 and CD8 staining. ICSs were classified as low, moderate, or high, based on the numbers of immune cells in the tumour core and invasive margin. We compared ICS groups determined with the hotspot and whole-section techniques. Associations between ICS and survival were analysed with Cox regression models, adjusted for sex, age, tumour stage, differentiation grade, perineural invasion, and resection radicality. In hotspot ICS analysis, 5-year overall survival rates for low, moderate, and high groups were 12.1%, 26.3%, and 26.8%, respectively (p = 0.193). In whole-section analyses, overall survival rates were 5.3%, 26.4%, and 43.8%, respectively (p = 0.030). In the adjusted Cox model, whole-section ICS groups were inversely associated with the overall mortality hazard ratio (HR): low, moderate, and high ICS groups had HRs of 1.00, 0.42 (95% CI 0.20-0.88), and 0.27 (95% CI 0.11-0.67), respectively. The number of immune cells per square millimetre in the tumour core and the invasive margin were significantly higher and had a wider range in hotspots than in whole-tissue sections. Accordingly, ICS could predict survival in patients with PDAC after surgery. Whole tissue section ICSs exhibited better prognostic value than hotspot ICSs. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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