Monitoring the Progression of Chronic Liver Damage in Rats Using [18F]PBR06.
Purpose: Accurate and rapid assessment of liver condition is the key to therapy for hepatitis patients. This study aim is to evaluate the peripheral benzodiazepine receptor (PBR) radioligand [18F]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline ([18F]PBR06) as a positron emission tomography (...
| Publicado en: | Molecular Imaging & Biology Vol. 21; no. 4; pp. 669 - 676 |
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| Autores principales: | , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Aug2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=137398400&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 137398400 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Aug2019 vid: 21 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 137398400 137398400 NLM30280292 137398400 10.1007/s11307-018-1282-z NLM30280292 137398400 ppf: 669 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Monitoring the Progression of Chronic Liver Damage in Rats Using [18F]PBR06. aug: au: Huang, Shuo Li, Chao Guo, Jun Zhang, Linlin Wu, Shuqi Wang, Hui Liang, Sheng affil: Department of Nuclear Medicine, Xin Hua Hospital Affiliated To Shanghai Jiao Tong University School, No. 1665, Kongjiang Street, Yangpu District, 200092, Shanghai, China sug: subj: Disease Progression Liver Amides Liver Pathology Radiopharmaceuticals RNA Receptors, Cell Surface Metabolism Aspartate Aminotransferase Blood Carrier Proteins Metabolism Alanine Aminotransferase Blood Rats Receptors, Cell Surface Animal Studies Tomography, Emission-Computed RNA Metabolism Liver Physiopathology Carrier Proteins Validation Studies Comparative Studies Evaluation Research Multicenter Studies ab: Purpose: Accurate and rapid assessment of liver condition is the key to therapy for hepatitis patients. This study aim is to evaluate the peripheral benzodiazepine receptor (PBR) radioligand [18F]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline ([18F]PBR06) as a positron emission tomography (PET) imaging tracer of chronic liver damage in a rat model.Procedures: A rat model of liver damage was made by bile duct ligation (BDL), which initiates a complex cascade of pathological events that leads to cholestasis and inflammation, eventually resulting in a severe fibrotic and severe hepatocyte injury. PET scanning, immunofluorescence staining, H&E staining, Masson's staining, and Quantitative real time polymerase chain reaction(qRT-PCR) were performed to elucidate the correlation among the expression level of PBR, radioactivity uptake, and the level of liver damage in the rat model of chronic inflammatory.Results: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL) increased after BDL and peaked in 1 week, then gradually decreased over the following 3 weeks, although still higher than that of control (all P < 0.05). Histological analysis demonstrated chronic severe damage in rat livers after BDL. The uptake of [18F]PBR06 increased in livers and was correlated with severity of liver damage. Further, the mRNA level of PBR was obviously higher compared to controls.Conclusions: [18F]PBR06 can serve as a sensitive probe to monitor the progression of inflammation in liver. [18F]PBR06 imaging may be used to accurately assess liver damage degree and guide the therapeutic schedule, especially for those patients with severe liver inflammation and damage but with normal or mildly elevated serum ALT and AST. As a non-invasive diagnostic application, [18F]PBR06 PET scan could be an alternative for patients who is unwilling to perform liver biopsy. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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