In vitro activity of voriconazole and amphotericin B against Candida albicans, Candida krusei, and Cryptococcus neoformans in human cerebrospinal fluid.
Purpose: Fungal central nervous system (CNS) infections show a high mortality rate and only a few antifungal agents are available to treat these infections. We hypothesize that the different biochemical properties of human cerebrospinal fluid (CSF) compared to the standard growth medium lead to the...
| Publicado en: | Infection Vol. 47; no. 4; pp. 565 - 571 |
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| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Aug2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=137665275&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 137665275 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03008126 NXO jtl: Infection issn: 03008126 maglogo: N pubinfo: dt: Aug2019 vid: 47 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 137665275 137665275 137665275 10.1007/s15010-019-01275-9 137665275 ppf: 565 ppct: 6 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: In vitro activity of voriconazole and amphotericin B against Candida albicans, Candida krusei, and Cryptococcus neoformans in human cerebrospinal fluid. aug: au: al Jalali, Valentin Sauermann, Robert Eberl, Sabine Zeitlinger, Markus affil: Department of Clinical Pharmacology, Vienna University Hospital, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria sug: subj: Voriconazole Pharmacodynamics Amphotericin B Pharmacodynamics Candida Drug Effects Candida Albicans Drug Effects Cryptococcus Drug Effects Voriconazole Cerebrospinal Fluid Amphotericin B Cerebrospinal Fluid Culture Media Human In Vitro Studies Biochemical Phenomena Antibiotics Therapeutic Use Voriconazole Therapeutic Use Amphotericin B Therapeutic Use Central Nervous System Fungal Infections Drug Therapy ab: Purpose: Fungal central nervous system (CNS) infections show a high mortality rate and only a few antifungal agents are available to treat these infections. We hypothesize that the different biochemical properties of human cerebrospinal fluid (CSF) compared to the standard growth medium lead to the altered activity of antifungal agents in CSF. We investigated the in vitro activity of two of these agents, i.e., amphotericin B (AmB) and voriconazole (VOR), against three different fungi in CSF in comparison to sabouraud-dextrose broth (SDB). Methods: CSF samples from patients who did not receive any antibiotics were collected. Time-kill curves were performed in CSF and SDB using static antibiotic concentrations of AmB and VOR against ATCC strains of Candida albicans, Candida krusei, and Cryptococcus neoformans. Results: In our experiments, both AmB and VOR showed superior activity in SDB compared to CSF. Nevertheless, AmB achieved fungicidal activity in CSF after 24 h against all test strains. Voriconazole only achieved fungistatic activity against C. albicans and C. neoformans in CSF. Conclusions: In summary, our data demonstrate that growth of fungal pathogens but even more importantly activity of antifungal agents against Candida and Cryptococcus species can differ significantly in CSF compared to the standard growth medium. Both findings should be taken into consideration when applying PK/PD simulations to fungal infections of the CNS. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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