Streamlining Quality Review of Mass Spectrometry Data in the Clinical Laboratory by Use of Machine Learning.

* Context.--Turnaround time and productivity of clinical mass spectrometric (MS) testing are hampered by timeconsuming manual review of the analytical quality of MS data before release of patient results. Objective.--To determine whether a classification model created by using standard machine learn...

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Publicado en:Archives of Pathology & Laboratory Medicine Vol. 143; no. 8; pp. 990 - 999
Autores principales: Min Yu, Bazydlo, Lindsay A. L., Bruns, David E., Harrison Jr., James H.
Formato: equations & formulas research tables/charts Journal Article
Publicado: College of American Pathologists Aug2019
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Aug2019
      vid: 143
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      pub: College of American Pathologists
      place: Northfield, Illinois
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        atl: Streamlining Quality Review of Mass Spectrometry Data in the Clinical Laboratory by Use of Machine Learning.
      aug:
        au:
          Min Yu
          Bazydlo, Lindsay A. L.
          Bruns, David E.
          Harrison Jr., James H.
        affil: Division of Laboratory Medicine, Department of Pathology, University of Virginia School of Medicine and Health System, Charlottesville
      sug:
        subj:
          Gas Chromatography-Mass Spectrometry
          Clinical Laboratories
          Machine Learning Utilization
          Algorithms
          Human
          Retrospective Design
          Cannabis Analysis
          Urinalysis
          Automation
      ab: * Context.--Turnaround time and productivity of clinical mass spectrometric (MS) testing are hampered by timeconsuming manual review of the analytical quality of MS data before release of patient results. Objective.--To determine whether a classification model created by using standard machine learning algorithms can verify analytically acceptable MS results and thereby reduce manual review requirements. Design.--We obtained retrospective data from gas chromatography-MS analyses of 11-nor-9-carboxy-delta- 9-tetrahydrocannabinol (THC-COOH) in 1267 urine samples. The data for each sample had been labeled previously as either analytically unacceptable or acceptable by manual review. The dataset was randomly split into training and test sets (848 and 419 samples, respectively), maintaining equal proportions of acceptable (90%) and unacceptable (10%) results in each set. We used stratified 10-fold cross-validation in assessing the abilities of 6 supervised machine learning algorithms to distinguish unacceptable from acceptable assay results in the training dataset. The classifier with the highest recall was used to build a final model, and its performance was evaluated against the test dataset. Results.--In comparison testing of the 6 classifiers, a model based on the Support Vector Machines algorithm yielded the highest recall and acceptable precision. After optimization, this model correctly identified all unacceptable results in the test dataset (100% recall) with a precision of 81%. Conclusions.--Automated data review identified all analytically unacceptable assays in the test dataset, while reducing the manual review requirement by about 87%. This automation strategy can focus manual review only on assays likely to be problematic, allowing improved throughput and turnaround time without reducing quality.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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