In Vivo 3D MRI Measurement of Tumour Volume in an Orthotopic Mouse Model of Prostate Cancer.
Prostate cancer (CaP) is the most commonly diagnosed cancer in males in western countries. Orthotopic implantation is considered as an ideal xenograft model for CaP study, and noninvasive measurement of tumor volume changes is important for monitoring responses to anticancer therapies. In this study...
| Publicado en: | Cancer Control: Journal of the Moffitt Cancer Center Vol. 26; no. 1 |
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| Autores principales: | , , , , , |
| Formato: | diagnostic images research tables/charts Journal Article |
| Publicado: |
Sage Publications Inc.
Jan2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=137819371&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 137819371 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10732748 1CJM jtl: Cancer Control: Journal of the Moffitt Cancer Center issn: 10732748 maglogo: Y pubinfo: dt: Jan2019 vid: 26 iid: 1 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 137819371 137819371 NLM31032634 137819371 10.1177/1073274819846590 NLM31032634 137819371 ppct: 1 formats: tig: atl: In Vivo 3D MRI Measurement of Tumour Volume in an Orthotopic Mouse Model of Prostate Cancer. aug: au: Ni, Jie Bongers, Andre Chamoli, Uphar Bucci, Joseph Graham, Peter Li, Yong affil: Cancer Care Centre, St George Hospital, Kogarah, New South Wales, Australia sug: subj: Magnetic Resonance Imaging Methods Prostatic Neoplasms Image Processing, Computer Assisted Methods Prostatic Neoplasms Drug Therapy Male Mice Luminescent Measurements Animals Models, Biological Cell Line, Tumor Prostatic Neoplasms Pathology Body Weights and Measures Animal Studies Interview Guides Male ab: Prostate cancer (CaP) is the most commonly diagnosed cancer in males in western countries. Orthotopic implantation is considered as an ideal xenograft model for CaP study, and noninvasive measurement of tumor volume changes is important for monitoring responses to anticancer therapies. In this study, the T2-weighted fast spin echo sequence magnetic resonance imaging (MRI) was performed on a CaP orthotopic non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mouse model weekly for 6 weeks post PC-3 CaP cell inoculation, and the fat signal was suppressed using a chemical shift-selective pulse. Subsequently, the MRI data were imported into the image processing software Avizo Standard and stacked into three-dimensional (3D) volumes. Our results demonstrate that MRI, combined with 3D reconstruction, is a feasible and sensitive method to assess tumor growth in a PC-3 orthotopic CaP mouse model and this established monitoring approach is promising for longitudinal observation of CaP xenograft development after anticancer therapy in vivo. Further investigation is needed to validate this protocol in a larger cohort of mice to generate enough statistical power. pubtype: Academic Journal doctype: diagnostic images research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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