TAAR1 levels and sub-cellular distribution are cell line but not breast cancer subtype specific.
Trace amine-associated receptors are G protein-coupled receptors of which TAAR1 is the most well-studied. Recently, Vattai et al. (J Cancer Res Clin Oncol 143:1637–1647 10.1007/s00432-017-2420-8, 2017) reported that expression of TAAR1 may be a marker of breast cancer (BC) survival, with a positive...
| Publicado en: | Histochemistry & Cell Biology Vol. 152; no. 2; pp. 155 - 167 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Aug2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=137849226&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 137849226 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: Aug2019 vid: 152 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 137849226 10.1007/s00418-019-01791-7 137849226 ppf: 155 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: TAAR1 levels and sub-cellular distribution are cell line but not breast cancer subtype specific. aug: au: Pitts, Mallory S. McShane, Josh N. Hoener, Marius C. Christian, Sherri L. Berry, Mark D. affil: Department of Biochemistry, Memorial University of Newfoundland, 232 Elizabeth Ave, A1B 3X9, St. John's, NL, Canada sug: ab: Trace amine-associated receptors are G protein-coupled receptors of which TAAR1 is the most well-studied. Recently, Vattai et al. (J Cancer Res Clin Oncol 143:1637–1647 10.1007/s00432-017-2420-8, 2017) reported that expression of TAAR1 may be a marker of breast cancer (BC) survival, with a positive correlation also suggested between TAAR1 expression and HER2 positivity. Neither a role for TAAR1 in breast tissue, nor in cancer, had previously been suspected. We, therefore, sought to provide independent validation and to further examine these putative relationships. First, a bioinformatic analysis on 58 total samples including normal breast tissue, BC-related cell lines, and tumour samples representing different BC sub-types found no clear correlation between TAAR1 mRNA levels and any BC subtype, including HER2 +. We next confirmed the bioinformatics data correlated to protein expression using a well validated anti-human TAAR1 antibody. TAAR1 mRNA levels correlated with the relative intensity of immunofluorescence staining in six BC cell lines (MCF-7, T47D, MDA-MB-231, SKBR3, MDA-MB-468, BT-474), but not in the MCF-10A immortalized mammary gland line, which had high mRNA but low protein levels. As expected, TAAR1 protein was intracellular in all cell lines. Surprisingly MCF-7, SKBR3, and MDA-MB-468 showed pronounced nuclear localization. The relative protein expression in MCF-7, MDA-MB-231, and MCF-10A lines was further confirmed by semi-quantitative flow cytometry. Finally, we demonstrate that the commercially available anti-TAAR1 antibody has poor selectivity, which likely explains the lack of correlation with the previous study. Therefore, while we clearly demonstrate variable expression and sub-cellular localization of TAAR1 across BC cell lines, we find no evidence for association with BC subtype. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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