Non-invasive screening of a microRNA-based dysregulation signature in oral cancer and oral potentially malignant disorders.
Introduction: We have previously shown that oral swirls are a robust source of microRNA protected by extracellular vesicles, potentially useful to detect oral squamous cell carcinoma (OSCC)-associated molecular aberration.Objectives: To study a developed dysregulation score and risk classification a...
| Publicado en: | Oral Oncology Vol. 96; pp. 113 - 121 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Pergamon Press - An Imprint of Elsevier Science
Sep2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=138101783&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 138101783 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13688375 JRJ jtl: Oral Oncology issn: 13688375 maglogo: N pubinfo: dt: Sep2019 vid: 96 pid: 2410 pub: Pergamon Press - An Imprint of Elsevier Science artinfo: ui: 138101783 138101783 NLM31422202 138101783 10.1016/j.oraloncology.2019.07.013 NLM31422202 138101783 ppf: 113 ppct: 8 formats: tig: atl: Non-invasive screening of a microRNA-based dysregulation signature in oral cancer and oral potentially malignant disorders. aug: au: Yap, T. Seers, C. Koo, K. Cheng, L. Vella, L.J. Hill, A.F. Reynolds, E. Nastri, A. Cirillo, N. McCullough, M. affil: Melbourne Dental School, University of Melbourne, Victoria, Australia sug: subj: RNA Mouth Neoplasms Carcinoma, Squamous Cell Middle Age Male Carcinoma, Squamous Cell Pathology Human Female Mouth Neoplasms Pathology Early Detection of Cancer Validation Studies Comparative Studies Evaluation Research Multicenter Studies Middle Aged: 45-64 years Male Female ab: Introduction: We have previously shown that oral swirls are a robust source of microRNA protected by extracellular vesicles, potentially useful to detect oral squamous cell carcinoma (OSCC)-associated molecular aberration.Objectives: To study a developed dysregulation score and risk classification algorithm based upon a panel of OSCC-associated microRNA in oral swirls from individuals with OSCC and oral potentially malignant disorders (OPMDs).Materials and Methods: An OSCC-associated panel of 5 microRNAs (miR-24; miR-21; miR-99a; let-7c; miR-100;) was quantified by qPCR in 190 individuals with and without mucosal abnormalities, including OSCC (n = 53) and OPMDs (n = 74). Each sample was analyzed using a developed dysregulation score (dSCORE) and risk classification algorithm, allocating a LOW- or HIGH-RISK score. The influence of demographic, systemic, oral health and mucosal disease factors on the developed test was analyzed.Results: MicroRNA for analysis can be predictably isolated from oral swirls sourced from individuals with a range of demographic, systemic and oral health findings. Utilizing the presence of HIGH-RISK identified OSCC patients with 86.8% sensitivity and 81.5% specificity. Older age and female gender were associated with higher dSCOREs and higher proportions of HIGH-RISK classification amongst individuals with no mucosal abnormalities. The dSCOREs for all subgroups of OPMDs were significantly different from the OSCC group.Conclusion: This is the first comparison of microRNA sourced from oral swirls from individuals with OPMDs with individuals with and without OSCC. A HIGH-RISK dysregulation signature was found to be accurate in indicating the presence of OSCC and exampled to parallel malignant transformation. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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