The Caenorhabditis elegans Transgenic Toolbox.
The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, ne...
| Publicado en: | Genetics Vol. 212; no. 4; pp. 959 - 991 |
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| Autores principales: | , |
| Formato: | Journal Article |
| Publicado: |
Oxford University Press / USA
Aug2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=138132281&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 138132281 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00166731 GNT jtl: Genetics issn: 00166731 maglogo: N pubinfo: dt: Aug2019 vid: 212 iid: 4 pid: 622 pub: Oxford University Press / USA artinfo: ui: 138132281 10.1534/genetics.119.301506 138132281 ppf: 959 ppct: 32 formats: tig: atl: The Caenorhabditis elegans Transgenic Toolbox. aug: au: Nance, Jeremy Frøkjær-Jensen, Christian affil: Helen L. and Martin S. Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York 10016 sug: subj: Nematodes Analysis Organisms, Genetically Modified Analysis Sequence Analysis Methods Gene Expression Evaluation DNA Analysis Clustered Regularly Interspaced Short Palindromic Repeats Analysis CRISPR-Associated Proteins Analysis ab: The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, neurobiology, and aging. Over the last decade, the C. elegans transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution. Here, we provide a comprehensive overview of transgenic methods in C. elegans, with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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