Novel perspectives in fetal biomarker implementation for the noninvasive prenatal testing.

Noninvasive prenatal testing (NIPT) utilizes cell-free fetal DNA (cffDNA) present in maternal peripheral blood to detect chromosomal abnormalities. The detection of 21-trisomy, 18-trisomy, and 13-trisomy in the fetus has become a common screening method during pregnancy and has been widely applied i...

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Publicado en:Critical Reviews in Clinical Laboratory Sciences Vol. 56; no. 6; pp. 374 - 393
Autores principales: Shi, Jiping, Zhang, Runling, Li, Jinming, Zhang, Rui
Formato: pictorial review tables/charts Journal Article
Publicado: Taylor & Francis Ltd Sep2019
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Sep2019
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      pub: Taylor & Francis Ltd
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        atl: Novel perspectives in fetal biomarker implementation for the noninvasive prenatal testing.
      aug:
        au:
          Shi, Jiping
          Zhang, Runling
          Li, Jinming
          Zhang, Rui
        affil: Peking University Fifth School of Clinical Medicine, National Center for Clinical Laboratories, National Center of Gerontology, Beijing Hospital, Beijing, China
      sug:
        subj:
          Biological Markers Blood
          Fetal Blood Analysis
          DNA
          Prenatal Diagnosis Methods
          Noninvasive Procedures
          Aneuploidy Diagnosis
          Polymorphism, Genetic
          Hereditary Diseases Diagnosis
          Genetic Screening Methods
          Attitude of Health Personnel
          Medical Practice, Evidence-Based
          Sensitivity and Specificity
          Predictive Value of Tests
          Diagnosis, Laboratory
          Practice Guidelines
          Fetus
          Physicians
          Scientists
          Research, Medical
          Information Resources
          Fetus, conception to birth
      ab: Noninvasive prenatal testing (NIPT) utilizes cell-free fetal DNA (cffDNA) present in maternal peripheral blood to detect chromosomal abnormalities. The detection of 21-trisomy, 18-trisomy, and 13-trisomy in the fetus has become a common screening method during pregnancy and has been widely applied in routine clinical testing because of its analytical and clinical validity. Currently, noninvasive prenatal testing involving copy number variations (CNVs) and other frequent single-gene disorders is being widely studied, and it plays an important and indispensable role in prenatal detection. The multiple approaches that have been reported and validated by various laboratories have different merits and limitations. Their clinical validity, utility, and application vary with different diseases. This review summarizes the principles, methods, advantages, and limitations of noninvasive prenatal testing for the detection of aneuploidy, CNVs and single-gene disorders. Before implementation of NIPT into clinical practice, a list of criteria that the application must meet is crucial. Essential parameters such as clinical sensitivity, clinical specificity, positive predictive value (PPV) and negative predictive value (NPV) are required to properly evaluate the clinical validity and utility of NIPT. We then discuss and analyze these clinical parameters and clinical application guidelines, providing physicians and scientists with feasible strategies and the latest research information.
      pubtype: Academic Journal
      doctype:
        pictorial
        review
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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