Next-generation sequencing for the clinical management of hepatitis C virus infections: does one test fits all purposes?
While the prospect of viral cure is higher than ever for individuals infected with the hepatitis C virus (HCV) due to ground-breaking progress in antiviral treatment, success rates are still negatively influenced by HCV's high genetic variability. This genetic diversity is represented in the circula...
| Publicado en: | Critical Reviews in Clinical Laboratory Sciences Vol. 56; no. 6; pp. 420 - 435 |
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| Autores principales: | , , , , , |
| Formato: | pictorial questions and answers review tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Sep2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=138399824&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 138399824 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10408363 1AV jtl: Critical Reviews in Clinical Laboratory Sciences issn: 10408363 maglogo: Y pubinfo: dt: Sep2019 vid: 56 iid: 6 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 138399824 138399824 138399824 10.1080/10408363.2019.1637394 138399824 ppf: 420 ppct: 15 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Next-generation sequencing for the clinical management of hepatitis C virus infections: does one test fits all purposes? aug: au: Cuypers, Lize Thijssen, Marijn Shakibzadeh, Arash Sabahi, Farzaneh Ravanshad, Mehrdad Pourkarim, Mahmoud Reza affil: Laboratory of Clinical and Epidemiological Virology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium sug: subj: Sequence Analysis Disease Management Hepatitis C Diagnosis Molecular Diagnostic Techniques Genotype Coinfection Drug Resistance, Microbial Genetic Techniques Hepatitis C Prevention and Control ab: While the prospect of viral cure is higher than ever for individuals infected with the hepatitis C virus (HCV) due to ground-breaking progress in antiviral treatment, success rates are still negatively influenced by HCV's high genetic variability. This genetic diversity is represented in the circulation of various genotypes and subtypes, mixed infections, recombinant forms and the presence of numerous drug resistant variants among infected individuals. Common misclassifications by commercial genotyping assays in combination with the limitations of currently used targeted population sequencing approaches have encouraged researchers to exploit alternative methods for the clinical management of HCV infections. Next-generation sequencing (NGS), a revolutionary and powerful tool with a variety of applications in clinical virology, can characterize viral diversity and depict viral dynamics in an ultra-wide and ultra-deep manner. The level of detail it provides makes it the method of choice for the diagnosis and clinical assessment of HCV infections. The sequence library provided by NGS is of a higher magnitude and sensitivity than data generated by conventional methods. Therefore, these technologies are helpful to guide clinical practice and at the same time highly valuable for epidemiological studies. The decreasing costs of NGS to determine genotypes, mixed infections, recombinant strains and drug resistant variants will soon make it feasible to employ NGS in clinical laboratories, to assist in the daily care of patients with HCV. pubtype: Academic Journal doctype: pictorial questions and answers review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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