Long non-coding RNA myocardial infarction associated transcript promotes epithelial-mesenchymal transition and is an independent risk factor for poor prognosis of tongue squamous cell carcinoma.

Background: Long non-coding RNA myocardial infarction associated transcript (MIAT) has been suspected to be associated with poor prognosis in several malignancies. This study aims to investigate the association between MIAT expression levels and prognosis of tongue squamous cell carcinoma (TSCC).Mat...

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Publicado en:Journal of Oral Pathology & Medicine Vol. 48; no. 8; pp. 720 - 728
Autores principales: Zhong, Waisheng, Xu, Zi, Wen, Senli, Xie, Tao, Wang, Fang, Wang, Qiwei, Chen, Jie
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell Sep2019
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Sep2019
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1111/jop.12892
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        atl: Long non-coding RNA myocardial infarction associated transcript promotes epithelial-mesenchymal transition and is an independent risk factor for poor prognosis of tongue squamous cell carcinoma.
      aug:
        au:
          Zhong, Waisheng
          Xu, Zi
          Wen, Senli
          Xie, Tao
          Wang, Fang
          Wang, Qiwei
          Chen, Jie
        affil: Department of Head and Neck Surgery, Hunan Cancer Hospital & The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha China
      sug:
        subj:
          Tongue Neoplasms Diagnosis
          Carcinoma, Squamous Cell Diagnosis
          RNA
          Male
          Cell Line, Tumor
          Genes
          Carcinoma, Squamous Cell
          Prognosis
          Middle Age
          Female
          Risk Factors
          Signal Transduction
          Tongue Neoplasms
          Funding Source
          Middle Aged: 45-64 years
          Male
          Female
      ab: Background: Long non-coding RNA myocardial infarction associated transcript (MIAT) has been suspected to be associated with poor prognosis in several malignancies. This study aims to investigate the association between MIAT expression levels and prognosis of tongue squamous cell carcinoma (TSCC).Materials and Methods: Expressions of MIAT in TSCC specimens, corresponding adjacent non-neoplastic tongue tissues, and serums collected from 116 TSCC patients were detected by Quantitative Real-Time PCR. Then, these patients were followed up for 12 months after discharge. MIAT was suppressed and upregulated in TSCC cells, and then cell invasion and epithelial-mesenchymal transition (EMT) markers were analyzed.Results: Myocardial infarction associated transcript expression level in TSCC specimen was upregulated compared to adjacent non-neoplastic tongue tissue and had a significant positive association with its level in serum. MIAT levels in both TSCC specimen and serum were correlated with cervical lymph node metastasis and histological grading of TSCC patients. Results of survival analysis showed that high expression levels of MIAT in both TSCC tissues and serums indicated higher mortality and shorter survival time of TSCC patients. Receiver operating characteristic curve analysis showed that there was no significant difference between MIAT in TSCC specimen and serum to differentiate death and non-death of TSCC patients. In vitro study showed that MIAT could induce invasion of TSCC cells by regulating expressions of EMT markers through activation of Wnt/β-catenin signaling pathway.Conclusion: Upregulated MIAT promoted EMT of TSCC cells through activation of Wnt/β-catenin signaling pathway and indicated a poor prognosis of TSCC, which may provide us a non-invasive approach to evaluate the prognosis of TSCC.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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