An Automatable Method for Determining Adequacy of Thyroid Fine-Needle Aspiration Samples.

Context.--Thyroid nodules are a common clinical problem. Cytologic evaluation via fine-needle aspiration is often employed in the diagnostic workup, and rapid on-site assessment of adequacy can help ensure an adequate sample is obtained. However, rapid on-site assessment of adequacy only examines pa...

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Publicado en:Archives of Pathology & Laboratory Medicine Vol. 143; no. 9; pp. 1084 - 1089
Autores principales: Schmolze, Daniel B., Fischer, Andrew H.
Formato: algorithm pictorial research tables/charts Journal Article
Publicado: College of American Pathologists Sep2019
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Sep2019
      vid: 143
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      pub: College of American Pathologists
      place: Northfield, Illinois
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        atl: An Automatable Method for Determining Adequacy of Thyroid Fine-Needle Aspiration Samples.
      aug:
        au:
          Schmolze, Daniel B.
          Fischer, Andrew H.
        affil: Department of Pathology, City of Hope National Medical Center, Duarte, California
      sug:
        subj:
          Thyroid Neoplasms Diagnosis
          Biopsy, Needle Methods
          Automation
          Sensitivity and Specificity
          Fluorescent Antibody Technique Methods
          Human
          Algorithms
          Staining and Labeling Methods
          Microscopy Methods
          Pathologists Psychosocial Factors
          Digital Imaging Methods
      ab: Context.--Thyroid nodules are a common clinical problem. Cytologic evaluation via fine-needle aspiration is often employed in the diagnostic workup, and rapid on-site assessment of adequacy can help ensure an adequate sample is obtained. However, rapid on-site assessment of adequacy only examines part of the sample, a part that may not then be available for ancillary testing. Moreover, the procedure is time-consuming and poorly reimbursed. Objective.--To develop an automatable fluorescence-based image analysis system for assessing the adequacy of thyroid fine-needle aspirations that uses the entire aspirated sample. Design.--There were 12 previously diagnosed cases that served as a training set, and 11 cases were used for validation of an image analysis algorithm. The samples were fluorescently stained and imaged using a fluorescent microscope. The images were assessed for adequacy by an image analysis algorithm. Following image analysis, a ThinPrep slide was prepared and blindly scored by a cytopathologist. The standard and computer-derived results were then compared. Results.--The algorithm was optimized using the 12 cases in the training set and then applied to the 11 test cases. A total of 8 of 8 adequate samples in the test group were correctly scored as adequate, and 2 of 3 cases that were inadequate were correctly scored as inadequate by the algorithm. One case was erroneously designated as not adequate by the algorithm. Conclusions.--Our results demonstrate the feasibility of automating thyroid adequacy assessment using a fluorescent labeling technique followed by computer image analysis.
      pubtype: Academic Journal
      doctype:
        algorithm
        pictorial
        research
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        Journal Article
      ougenre: Article
    language: English
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