Bleeding peptic ulcers and presence of Helicobacter pylori by various tests: a case-control study.

Background: Virulence factors of Helicobacter pylori are associated with peptic ulcer disease and may be also associated with bleeding peptic ulcers (BPU).Aim: To determine whether H. pylori and/or the cytotoxin-associated gene (cagA) can increase the risk of bleeding in peptic ulcers.Patients: Sixt...

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Publicado en:European Journal of Gastroenterology & Hepatology Vol. 14; no. 10; pp. 1113 - 1119
Autores principales: Castillo-Rojas, Gonzalo, Ballesteros, M Arturo, Ponce de León, Sergio, Morales-Espinosa, Rosario, Cravioto, Alejandro, López-Vidal, Yolanda
Formato: research Journal Article
Publicado: Lippincott Williams & Wilkins 2002 Oct
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2002 Oct
      vid: 14
      iid: 10
      pid: 5086
      pub: Lippincott Williams & Wilkins
      place: Baltimore, Maryland
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        10.1097/00042737-200210000-00012
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        atl: Bleeding peptic ulcers and presence of Helicobacter pylori by various tests: a case-control study.
      aug:
        au:
          Castillo-Rojas, Gonzalo
          Ballesteros, M Arturo
          Ponce de León, Sergio
          Morales-Espinosa, Rosario
          Cravioto, Alejandro
          López-Vidal, Yolanda
        affil: Universidad Nacional Autónoma de México, Facultad de Medicina, Programa de Inmunología Molecular Microbiana, Edif. de investigación, 4to piso, México DF, CP 04510, México
      sug:
        subj:
          Helicobacter Pylori
          Duodenal Ulcer Microbiology
          Antigens, Bacterial
          Helicobacter Infections Complications
          Peptic Ulcer Microbiology
          Bacterial Proteins Analysis
          Male
          Prospective Studies
          Case Control Studies
          Immunoglobulins Blood
          Helicobacter Infections Blood
          Helicobacter Infections
          RNA
          RNA Analysis
          Middle Age
          Female
          Human
          Polymerase Chain Reaction Methods
          Regression
          Endoscopy, Gastrointestinal Methods
          Validation Studies
          Comparative Studies
          Evaluation Research
          Multicenter Studies
          Clinical Assessment Tools
          Middle Aged: 45-64 years
          Male
          Female
      ab: Background: Virulence factors of Helicobacter pylori are associated with peptic ulcer disease and may be also associated with bleeding peptic ulcers (BPU).Aim: To determine whether H. pylori and/or the cytotoxin-associated gene (cagA) can increase the risk of bleeding in peptic ulcers.Patients: Sixty-seven patients were studied. Thirty had BPU, 20 had non-bleeding peptic ulcers (NBPU), and 17 were control subjects (NPU).Methods: The prevalence of H. pylori was assessed by the urease fast test, histological examination, serology, and 16S ribosomal RNA and cagA gene amplification by polymerase chain reaction (PCR).Results: Histology and PCR showed greater sensitivity for diagnosis of H. pylori under bleeding circumstances when compared with other tests. Association of H. pylori was greater in the NBPU group (odds ratio [OR] 4.91, P = 0.06) than in the BPU group (OR 1.27, P = NS) when compared with the control group. When the BPU and NBPU groups were compared, H. pylori was found more often in the NBPU group (OR 0.26, P < 0.10 ). The cagA-positive gene showed a similar distribution in the three groups. The titres for anti-CagA immunoglobulin A (IgA) antibodies were higher in NBPU patients (83%) than in BPU or control patients. Furthermore, anti-urease immunoglobulin G (IgG) was detected more frequently among BPU and NBPU patients.Conclusions: NBPU patients had the highest prevalence of H. pylori by PCR. It seems unlikely that either H. pylori or the cagA-positive gene act as significant risk factors for bleeding in peptic ulcers. The lower prevalence of the microorganism among patients who bleed cannot be explained as an artificial finding.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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