Involvement of EP2 and EP4 Receptors in Eosinophilic Esophagitis: A Pilot Study.
Background: The prostaglandin D2 receptor DP2 has been implicated in eosinophil infiltration and the development of eosinophilic esophagitis (EoE).Aims and Methods: In this study, we investigated an involvement of PGE2 (EP1-EP4) and PGD2 (DP1) receptors in EoE by measuring their expression in periph...
| Publicado en: | Digestive Diseases & Sciences Vol. 64; no. 10; pp. 2806 - 2815 |
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| Autores principales: | , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Oct2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=138611196&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 138611196 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01632116 1VP jtl: Digestive Diseases & Sciences issn: 01632116 maglogo: N pubinfo: dt: Oct2019 vid: 64 iid: 10 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 138611196 138611196 143940114 NLM30989466 138611196 10.1007/s10620-019-05623-5 NLM30989466 138611196 ppf: 2806 ppct: 9 formats: tig: atl: Involvement of EP2 and EP4 Receptors in Eosinophilic Esophagitis: A Pilot Study. aug: au: Durchschein, Franziska Eherer, Andreas Grill, Magdalena Sturm, Eva M. Pommer, Veronika Langner, Cord Högenauer, Christoph Schicho, Rudolf affil: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria sug: subj: Receptors, Cell Surface Analysis Eosinophilic Esophagitis Pathology Eosinophils Drug Effects Receptors, Cell Surface Eosinophils Metabolism Eosinophilic Esophagitis Metabolism Eosinophilic Esophagitis Blood Prostaglandins, Synthetic Pharmacodynamics Vasodilator Agents Analogs and Derivatives Human Vasodilator Agents Pharmacodynamics Immunohistochemistry Cell Physiology Ethers Pharmacodynamics Pilot Studies Cells, Cultured Cell Migration Assays Methods Validation Studies Comparative Studies Evaluation Research Multicenter Studies Arthritis Impact Measurement Scales Funding Source ab: Background: The prostaglandin D2 receptor DP2 has been implicated in eosinophil infiltration and the development of eosinophilic esophagitis (EoE).Aims and Methods: In this study, we investigated an involvement of PGE2 (EP1-EP4) and PGD2 (DP1) receptors in EoE by measuring their expression in peripheral blood eosinophils and esophageal mucosal biopsies of EoE patients and by performing migration and adhesion assays with eosinophils from healthy donors.Results: Expression of EP2 and EP4, but not EP1 and EP3, was decreased in blood eosinophils of patients with EoE vs. control subjects. Adhesion of eosinophils to esophageal epithelial cells was decreased by EP2 receptor agonist butaprost and EP4 agonist ONO-AE1-329, whereas DP1 agonist BW245C increased adhesion. In chemotaxis assays with supernatant from human esophageal epithelial cells, only ONO-AE1-329 but not butaprost or BW245C inhibited the migration of eosinophils. Expression of EP and DP receptors in epithelial cells and eosinophils was detected in sections of esophageal biopsies from EoE patients by immunohistochemistry. qPCR of biopsies from EoE patients revealed that gene expression of EP4 and DP1 was the highest among PGE2 and PGD2 receptors. Esophageal epithelial cells in culture showed high gene expression for EP2 and EP4. Activation of EP2 and EP4 receptors decreased barrier integrity of esophageal epithelial cells in impedance assays.Conclusions: Activation of EP2 and EP4 receptors may inhibit eosinophil recruitment to the esophageal mucosa. However, their activation could negatively affect esophageal barrier integrity suggesting that eosinophilic rather than epithelial EP2 and EP4 have a protective role in EoE. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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