Assessment of angiogenesis in rabbit orthotropic liver tumors using three-dimensional dynamic contrast-enhanced ultrasound compared with two-dimensional DCE-US.
Objectives: To evaluate quantitative three-dimensional (3D) dynamic contrast-enhanced ultrasound (DCE-US) in the assessment of tumor angiogenesis using an orthotropic liver tumor model.Methods: Nine New Zealand white rabbits with liver orthotropic VX2 tumors were established and imaged by two-dimens...
| Published in: | Japanese Journal of Radiology Vol. 37; no. 10; pp. 701 - 710 |
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| Main Authors: | , , , , , , , , , , , , |
| Format: | research Journal Article |
| Published: |
Springer Nature
Oct2019
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=138884763&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 138884763 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 18671071 AUCM jtl: Japanese Journal of Radiology issn: 18671071 maglogo: N pubinfo: dt: Oct2019 vid: 37 iid: 10 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 138884763 138884763 144099983 NLM31401722 138884763 10.1007/s11604-019-00861-z NLM31401722 138884763 ppf: 701 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Assessment of angiogenesis in rabbit orthotropic liver tumors using three-dimensional dynamic contrast-enhanced ultrasound compared with two-dimensional DCE-US. aug: au: Zheng, Qiao Zhang, Jian-chao Wang, Zhu Ruan, Si-Min Li, Wei Pan, Fu-Shun Chen, Li-Da Zhang, Yu-Chen Wu, Wen-Xin Xie, Xiao-Yan Lu, Ming-De Shan, Quan-Yuan Wang, Wei affil: Department of Medical Ultrasonics, Institute of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital of Sun Yat-Sen University, No. 58 Zhongshan Road 2, 510080, Guangzhou, People's Republic of China sug: subj: Neovascularization, Pathologic Liver Neoplasms Imaging, Three-Dimensional Methods Image Enhancement Methods Contrast Media Ultrasonography Methods Neovascularization, Pathologic Pathology Models, Biological Liver Human Animal Studies Liver Pathology Liver Neoplasms Pathology Rabbits Reproducibility of Results Validation Studies Comparative Studies Evaluation Research Multicenter Studies ab: Objectives: To evaluate quantitative three-dimensional (3D) dynamic contrast-enhanced ultrasound (DCE-US) in the assessment of tumor angiogenesis using an orthotropic liver tumor model.Methods: Nine New Zealand white rabbits with liver orthotropic VX2 tumors were established and imaged by two-dimensional (2D) and 3D DCE-US after SonoVue® bolus injections. The intraclass correlation coefficients of perfusion parameters, including peak intensity (PI), mean transit time, time to peak, and area under the curve, were calculated based on time-intensity curve. The percentage area of microvascular (PAMV) and the expression of vascular endothelial growth factor (VEGF) were both evaluated by immunohistochemical analysis and weighted by the tumor activity area ratio. Correlations between quantitative and histologic parameters were analyzed.Results: The reproducibility of 3D DCE-US quantitative parameters was excellent (ICC 0.91-0.99); but only PI showed high reproducibility (ICC 0.97) in 2D. None of the parameters of quantitative 2D DCE-US were significantly correlated with weighted PAMV or VEGF. For 3D DCE-US, there was a positive correlation between PI and weighted PAMV (r = 0.74, P = 0.04) as well as VEGF (r = 0.79, P = 0.02).Conclusion: Quantitative parameters of 3D DCE-US show feasibility, higher reproducibility and accuracy for the assessment of tumor angiogenesis using an orthotropic liver tumor model compared with 2D DCE-US. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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