Intestinal Microbial Products From Alcohol‐Fed Mice Contribute to Intestinal Permeability and Peripheral Immune Activation.
Background: Alcohol use causes significant disruption of intestinal microbial communities, yet exactly how these dysbiotic communities interact with the host is unclear. We sought to understand the role of microbial products associated with alcohol dysbiosis in mice on intestinal permeability and im...
| Publicado en: | Alcoholism: Clinical & Experimental Research Vol. 43; no. 10; pp. 2122 - 2134 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Oct2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=138990978&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 138990978 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01456008 IQE jtl: Alcoholism: Clinical & Experimental Research issn: 01456008 maglogo: Y pubinfo: dt: Oct2019 vid: 43 iid: 10 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 138990978 138990978 138990978 10.1111/acer.14176 138990978 ppf: 2122 ppct: 12 formats: tig: atl: Intestinal Microbial Products From Alcohol‐Fed Mice Contribute to Intestinal Permeability and Peripheral Immune Activation. aug: au: Samuelson, Derrick R. Gu, Min Shellito, Judd E. Molina, Patricia E. Taylor, Christopher M. Luo, Meng Welsh, David A. affil: Section of Pulmonary/Critical Care and Allergy/Immunology, Department of Internal Medicine, Louisiana State University Health Sciences Center, New Orleans Louisiana sug: subj: Binge Drinking Metabolism Binge Drinking Microbiology Binge Drinking Immunology Intestines Metabolism Gut Microbiota Metabolism Intestines Immunology Gut Microbiota Immunology Animal Studies Mice In Vitro Studies Models, Biological Binge Drinking Epithelial Cells Electric Stimulation Methods Flow Cytometry Blood Cells Analysis Antigens, Surface Analysis Descriptive Statistics ab: Background: Alcohol use causes significant disruption of intestinal microbial communities, yet exactly how these dysbiotic communities interact with the host is unclear. We sought to understand the role of microbial products associated with alcohol dysbiosis in mice on intestinal permeability and immune activation in an in vitro model system. Methods: Microbiota samples from binge‐on‐chronic alcohol‐fed and pair‐fed male and female mice were cultured in Gifu Anaerobic Broth for 24 hours under anaerobic conditions. Live/whole organisms were removed, and microbial products were collected and added to human peripheral blood mononuclear cells (PBMCs) or polarized C2BBe1 intestinal epithelial monolayers. Following stimulation, transepithelial electrical resistance (TEER) was measured using a volt/ohm meter and immune activation of PBMC was assessed via flow cytometry. Results: Microbial products from male and female alcohol‐fed mice significantly decreased TEER (mean percentage change from baseline alcohol‐fed 0.86 Ω/cm2 vs. pair‐fed 1.10 Ω/cm2) compared to microbial products from control mice. Following ex vivo stimulation, immune activation of PBMC was assessed via flow cytometry. We found that microbial products from alcohol‐fed mice significantly increased the percentage of CD38+ CD4+ (mean alcohol‐fed 17.32% ± 0.683% standard deviation (SD) vs. mean pair‐fed 14.2% ± 1.21% SD, p < 0.05) and CD8+ (mean alcohol‐fed 20.28% ± 0.88% SD vs. mean pair‐fed 12.58% ± 3.59% SD, p < 0.05) T cells. Conclusions: Collectively, these data suggest that microbial products contribute to immune activation and intestinal permeability associated with alcohol dysbiosis. Further, utilization of these ex vivo microbial product assays will allow us to rapidly assess the impact of microbial products on intestinal permeability and immune activation and to identify probiotic therapies to ameliorate these defects. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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