Immunoglobulin-Like Transcript 5 Inhibits Macrophage-Mediated Bacterial Killing and Antigen Presentation During Sepsis.
Background: Immunosuppression contributes to the mortality of sepsis. However, the underlying mechanism remains unclear.Methods: In the present study, we investigated the role of inhibitory receptor immunoglobulin-like transcript 5 (ILT5) in sepsis. We first screened the expression of ILT family mem...
| Publicado en: | Journal of Infectious Diseases Vol. 220; no. 10; pp. 1688 - 1700 |
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| Autores principales: | , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Oxford University Press / USA
11/15/2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=139034333&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 139034333 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00221899 1YU jtl: Journal of Infectious Diseases issn: 00221899 maglogo: N pubinfo: dt: 11/15/2019 vid: 220 iid: 10 pid: 622 pub: Oxford University Press / USA artinfo: ui: 139034333 139034333 NLM31250008 139034333 10.1093/infdis/jiz319 NLM31250008 139034333 ppf: 1688 ppct: 12 formats: tig: atl: Immunoglobulin-Like Transcript 5 Inhibits Macrophage-Mediated Bacterial Killing and Antigen Presentation During Sepsis. aug: au: Ming, Siqi Li, Musheng Wu, Minhao Zhang, Jianhui Zhong, Haibo Chen, Junyang Huang, Yaopan Bai, Jun Huang, Li Chen, Juan Lin, Quanshi Liu, Jiao Tao, Jianping He, Duanman Huang, Xi affil: Program of Infection and Immunity, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhongshan School of Medicine, Sun Yat-sen University, Zhuhai, China sug: subj: Sepsis Pathology Immunity, Cellular Antigens, Surface Metabolism Bacterial Infections Pathology Macrophages Immunology Bacteria Immunology Receptors, Cell Surface Metabolism Adult Adolescence Tissue Culture Techniques Human Child, Preschool T Lymphocytes Immunology Cell Differentiation Mice Animal Studies Middle Age Child Cell Physiology Young Adult Male Female Validation Studies Comparative Studies Evaluation Research Multicenter Studies Adult: 19-44 years Adolescent: 13-18 years Child, Preschool: 2-5 years Middle Aged: 45-64 years Child: 6-12 years Male Female ab: Background: Immunosuppression contributes to the mortality of sepsis. However, the underlying mechanism remains unclear.Methods: In the present study, we investigated the role of inhibitory receptor immunoglobulin-like transcript 5 (ILT5) in sepsis. We first screened the expression of ILT family members, and we found that ILT5 was dramatically up-regulated in the peripheral blood mononuclear cells from sepsis patients versus healthy donors.Results: Knockdown of ILT5 by small interfering ribonucleic acid increased bacterial killing and reactive oxygen species production in THP-1 and RAW264.7 cells. Moreover, ILT5-expressing monocytes/macrophages exhibited lower expression of antigen-presenting molecules including major histocompatibility complex-II and CD80. In the in vitro coculture system with monocytes/macrophages, blockage of ILT5 facilitated Th1 proliferation and differentiation of CD4+ T cells. Furthermore, in vivo experiments demonstrated that pretreatment with ILT5 blocking peptide improved the survival and pulmonary pathology of septic mice.Conclusions: Together, our study identified ILT5 as an immunosuppressive regulator during sepsis, which may provide potential therapeutic strategy for sepsis. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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