Determinate root development in the halted primary root1 mutant of Arabidopsis correlates with death of root initial cells and an enhanced auxin response.
The transit from indeterminate to determinate root developmental program compromises growth and causes the differentiation of the meristem, but a direct link between this process with auxin signaling and/or viability of initial cells remains untested. Here, through the isolation and characterization...
| Publicado en: | Protoplasma Vol. 256; no. 6; pp. 1657 - 1667 |
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| Autores principales: | , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Nov2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=139390313&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 139390313 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: Nov2019 vid: 256 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 139390313 144064891 10.1007/s00709-019-01409-8 139390313 ppf: 1657 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Determinate root development in the halted primary root1 mutant of Arabidopsis correlates with death of root initial cells and an enhanced auxin response. aug: au: Raya-González, Javier Ortiz-Castro, Randy López-Bucio, José affil: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio A1', Ciudad Universitaria, C. P., 58030, Morelia, Michoacán, Mexico sug: ab: The transit from indeterminate to determinate root developmental program compromises growth and causes the differentiation of the meristem, but a direct link between this process with auxin signaling and/or viability of initial cells remains untested. Here, through the isolation and characterization of the halted primary root1 (hpr1) mutant of Arabidopsis, which develops primary and lateral roots with genetically stable determinate growth after germination, we show that the differentiation of the root meristem correlates with enhanced auxin responsiveness and is preceded by the death of provasculature initial cells in both primary and lateral roots. Supplementation of indole-3-acetic acid causes both a dose-dependent repression of primary root growth and an induction of DR5:uidA expression in wild-type seedlings, and these effects were exacerbated in hpr1 mutants. The damage of provasculature initial cells in the root of hpr1 mutants occurred at earlier times than the full differentiation of the meristem, and correlates with a reduced expression domain of CycB1:uidA and PRZ:uidA. Thus, HPR1 plays critical functions for stem cell maintenance, auxin homeostasis, cell division in the meristem, and indeterminate root growth. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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