RNA-sequencing in ophthalmology research: considerations for experimental design and analysis.
High-throughput, massively parallel sequence analysis has revolutionized the way that researchers design and execute scientific investigations. Vast amounts of sequence data can be generated in short periods of time. Regarding ophthalmology and vision research, extensive interrogation of patient sam...
| Publicado en: | Therapeutic Advances in Ophthalmology Vol. 11 |
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| Autores principales: | , |
| Formato: | pictorial review tables/charts Journal Article |
| Publicado: |
Sage Publications Inc.
2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=139431078&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 139431078 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 25158414 LAW0 jtl: Therapeutic Advances in Ophthalmology issn: 25158414 maglogo: Y pubinfo: dt: 2019 vid: 11 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 139431078 139431078 139431078 10.1177/2515841419835460 139431078 ppct: 1 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: RNA-sequencing in ophthalmology research: considerations for experimental design and analysis. aug: au: Owen, Nicholas Moosajee, Mariya affil: Development, Ageing and Disease Theme, UCL Institute of Ophthalmology, University College London, London, UK sug: subj: Research, Medical Ophthalmology Bioinformatics Sequence Analysis Methods RNA Analysis Gene Expression Evaluation Eye Diseases Diagnosis DNA Analysis Mutation Evaluation Eye Diseases Familial and Genetic Gene Expression Profiling ab: High-throughput, massively parallel sequence analysis has revolutionized the way that researchers design and execute scientific investigations. Vast amounts of sequence data can be generated in short periods of time. Regarding ophthalmology and vision research, extensive interrogation of patient samples for underlying causative DNA mutations has resulted in the discovery of many new genes relevant to eye disease. However, such analysis remains functionally limited. RNA-sequencing accurately snapshots thousands of genes, capturing many subtypes of RNA molecules, and has become the gold standard for transcriptome gene expression quantification. RNA-sequencing has the potential to advance our understanding of eye development and disease; it can reveal new candidates to improve our molecular diagnosis rates and highlight therapeutic targets for intervention. But with a wide range of applications, the design of such experiments can be problematic, no single optimal pipeline exists, and therefore, several considerations must be undertaken for optimal study design. We review the key steps involved in RNA-sequencing experimental design and the downstream bioinformatic pipelines used for differential gene expression. We provide guidance on the application of RNA-sequencing to ophthalmology and sources of open-access eye-related data sets. pubtype: Academic Journal doctype: pictorial review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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