Myosin Heavy Chain-Associated RNA Transcripts Promotes Gastric Cancer Progression Through the miR-4529-5p/ROCK2 Axis.
Background and Aim: Characterization of genetic aberrations provides novel strategies for diagnosis and treatment of gastric cancer. Accumulating evidence has shown the involvement of long non-coding RNA (lncRNA) in the pathology of gastric cancer, especially in proliferation and metastasis. The aim...
| Publicado en: | Digestive Diseases & Sciences Vol. 64; no. 12; pp. 3539 - 3549 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Dec2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=139691378&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 139691378 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01632116 1VP jtl: Digestive Diseases & Sciences issn: 01632116 maglogo: N pubinfo: dt: Dec2019 vid: 64 iid: 12 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 139691378 139691378 144186938 NLM31273599 139691378 10.1007/s10620-019-05708-1 NLM31273599 139691378 ppf: 3539 ppct: 10 formats: tig: atl: Myosin Heavy Chain-Associated RNA Transcripts Promotes Gastric Cancer Progression Through the miR-4529-5p/ROCK2 Axis. aug: au: Sun, Xiaoli Zhang, Xinwu Chen, Shuo Fan, Meng Ma, Shuangyu Zhai, Hongjun affil: Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, No 157 Xinwu Road, 710004, Xi'an, China sug: subj: Apoptosis Phosphotransferases Cell Physiology Stomach Neoplasms RNA Mice Gastric Mucosa Animal Studies Cell Movement Neoplasm Invasiveness Muscle Proteins RNA Metabolism Cell Line Cell Line, Tumor Human Genetic Techniques Stomach Neoplasms Metabolism Phosphotransferases Metabolism Stomach Neoplasms Pathology Validation Studies Comparative Studies Evaluation Research Multicenter Studies Clinical Assessment Tools Scales ab: Background and Aim: Characterization of genetic aberrations provides novel strategies for diagnosis and treatment of gastric cancer. Accumulating evidence has shown the involvement of long non-coding RNA (lncRNA) in the pathology of gastric cancer, especially in proliferation and metastasis. The aim of this study was to delineate the role of myosin heavy chain-associated RNA transcripts (MHRT), a heart-specific lncRNA, in gastric cancer and to understand the correlation between MHRT, miR-4529-5p, and ROCK2.Methods: To study expression level of MHRT, clinical gastric cancer samples, gastric cancer cell lines, adjacent normal tissues, and gastric epithelial cell lines were used. Additionally, apoptosis, proliferation, and invasion of gastric cancer cells were studied with or without downregulation of MHRT and miR-4529-5p.Results: We identified that MHRT was ectopically expressed in gastric cancer tissues and cell lines. Interestingly, similar to the anti-apoptotic role of MHRT in cardiomyocytes, our data illustrated that MHRT inhibits apoptosis of gastric cancer cells. Moreover, we found that MHRT promotes proliferation and invasion of gastric cancer cells in vitro. Importantly, our data revealed that MHRT regulates the expression of miR-4529-5p via direct binding. Additionally, functional experiments illustrated that miR-4529-5p is particularly responsible for MHRT-mediated regulation of apoptosis. Besides, ROCK2 was identified as a downstream target of miR-4529-5p. Additionally, upregulated MHRT promotes the expression of ROCK2 by inhibiting miR-4529-5p.Conclusion: Our data illustrated a MHRT/miR-4529-5p/ROCK2 regulatory axis that contributes to the tumorigenesis of gastric cancer and provided potential therapeutic targets for precise gastric cancer treatment. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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