Accuracy of electromagnetic models to estimate cardiomyocyte membrane polarization.
External electric fields (E) induce a spatially heterogeneous variation in the membrane potential (ΔVm) of cardiomyocytes that, if sufficiently large, results in an action potential and contraction. Insights into the phenomenon of ΔVm induction by E have been classically gained with electromagnetic...
| Publicado en: | Medical & Biological Engineering & Computing Vol. 57; no. 12; pp. 2617 - 2628 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Dec2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=140034790&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 140034790 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01400118 PO0 jtl: Medical & Biological Engineering & Computing issn: 01400118 maglogo: N pubinfo: dt: Dec2019 vid: 57 iid: 12 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 140034790 140034790 NLM31667705 10.1007/s11517-019-02054-2 NLM31667705 140034790 ppf: 2617 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Accuracy of electromagnetic models to estimate cardiomyocyte membrane polarization. aug: au: Milan, Hugo F. M. Bassani, Rosana A. Santos, Luiz E. C. Almeida, Antonio C. G. Bassani, José W. M. affil: Department of Biological and Environmental Engineering, Cornell University, 321 Riley-Robb Hall, 14850, Ithaca, NY, USA sug: subj: Cells Membrane Potentials Physiology Cell Membrane Physiology Animals Electromagnetic Fields Rats Male Male ab: External electric fields (E) induce a spatially heterogeneous variation in the membrane potential (ΔVm) of cardiomyocytes that, if sufficiently large, results in an action potential and contraction. Insights into the phenomenon of ΔVm induction by E have been classically gained with electromagnetic models due to the lack of adequate experimental approaches. However, it is not clear yet how reliable these models are. To assess the accuracy of commonly used models, a reference 3D numerical model for cardiomyocytes (NMReal) was developed, consisting of the cell membrane shell reconstructed from rendered confocal microscopy images of freshly isolated ventricular myocytes. NMReal was used to estimate the E-induced maximum ΔVm values (ΔVmax), which were compared with estimates from seven other electromagnetic models. Accurate ΔVmax estimates (average error < 2%) were obtained with a less complex 3D model (NM3D) based on the extruded 2D image of the cell longitudinal section. Acceptable ΔVmax estimates (average error < 5%) were obtained with the prolate spheroid analytical model (PSAM) when the angle of E incidence and the cell major axis was < 30°. In this case, PSAM, a much simpler model requiring only the measurement of the longitudinal and transversal cell dimensions, can be a suitable alternative for ΔVmax calculation. Graphical abstract (A) Confocal images of the cell were used to reconstruct the realistic geometry of cardiomyocytes (NMReal). (B) NMReal was used to estimate the maximum variation in the transmembrane potential (ΔVmax) induced by an external electric field (E) applied at different angles with respect to the cell major axis. Plus (anode) and minus (cathode) signs indicate electrode position (E direction is from minus to plus). (C) Relative error (vs. NMReal) of ΔVmax estimation with simplified electromagnetic models, presented in descending order of accuracy (left-to-right, top-to-bottom). NM2D: 2D numerical model based on the longitudinal cell image; NM3D: numerical model based on the z extrusion of NM2D; EAM, PSAM, and CAM: ellipsoidal, prolate spheroidal, and cylindrical analytical models, respectively; PNM and CNM: parallelepipedal and cylindrical numerical models, respectively. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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