Effect of patchouli alcohol on macrophage mediated Helicobacter pylori digestion based on intracellular urease inhibition.
Background: Helicobacter pylori infects almost half of the world population and is listed as a type I carcinoma factor since 1994. Pogostemon cablin (Blanco) Benth. (Labiatae) has been used to treat gastro-intestinal diseases for thousands of years in many east Asian countries, and the key ingredien...
| Published in: | Phytomedicine Vol. 65 |
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| Main Authors: | , , , , , , , , , , , |
| Format: | Journal Article |
| Published: |
Elsevier GmbH, Urban & Fischer Verlag
Dec2019
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=140096094&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 140096094 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09447113 NXH jtl: Phytomedicine issn: 09447113 maglogo: N pubinfo: dt: Dec2019 vid: 65 pid: 24997 pub: Elsevier GmbH, Urban & Fischer Verlag place: London, <Blank> artinfo: ui: 140096094 140096094 NLM31568921 10.1016/j.phymed.2019.153097 NLM31568921 140096094 ppct: 1 formats: tig: atl: Effect of patchouli alcohol on macrophage mediated Helicobacter pylori digestion based on intracellular urease inhibition. aug: au: Lian, DW Xu, YF Deng, QH Lin, XM Huang, B Xian, SX Huang, P Lian, D W Xu, Y F Deng, Q H Lin, X M Xian, S X affil: The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510407, PR China sug: subj: Helicobacter Infections Drug Therapy Hydrocarbons Pharmacodynamics Macrophages Drug Effects Helicobacter Pylori Drug Effects Urease Antagonists and Inhibitors Helicobacter Pylori Bacterial Proteins Phagocytosis Drug Effects Enzyme Inhibitors Pharmacodynamics Urease Macrophages Microbiology Macrophages Metabolism Mice Genes Drug Effects Animals Helicobacter Infections Microbiology Bacterial Proteins Antagonists and Inhibitors Antibiotics Pharmacodynamics ab: Background: Helicobacter pylori infects almost half of the world population and is listed as a type I carcinoma factor since 1994. Pogostemon cablin (Blanco) Benth. (Labiatae) has been used to treat gastro-intestinal diseases for thousands of years in many east Asian countries, and the key ingredient, patchouli alcohol (PA), has been observed to exert anti-H. pylori and anti-urease activities.Purpose: We investigated the effect of PA on H. pylori urease and its subsequent influence on macrophage phagosome maturation and function.Methods: In H. pylori experiment, the berthelot method and pH shock assay were adopted to evaluate the effect of PA on extracellular and intracellular H. pylori urease. And then, Q-PCR and Western blot were carried out to analyze the alterations in the expression of urease-related genes and proteins after PA treatment. In the H. pylori and macrophage cell (RAW264.7) co-culture experiment, the effects of PA on H. pylori-induced phagocytosis and intracellular killing of RAW264.7 were investigated using gentamycin protection assay, and the underlying mechanism was explored by immunofluorescence.Results: PA at 25 and 50 μM inhibited intracellular H. pylori urease activity but not isolated urease by down-regulating the gene expression levels of ureB, ureE, ureI and nixA and reducing the protein expression level of UreB, thereby inhibiting the acid resistance of H. pylori. PA also recovered the function of macrophage bacterial digestion, and prior treatment with ammonium chloride inhibited the efficacy of PA.Conclusion: PA suppressed intracellular H. pylori urease function and maturation, which increased macrophage digestion ability. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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