Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH against Helicobacter pylori.
The presence of Helicobacter pylori in the oral cavity has been associated to the failure of antimicrobial therapy in patients with gastrointestinal infection and the development of oral diseases. However, it has been reported that the maintenance of good oral hygiene can improve the therapeutic suc...
| Publicado en: | BioMed Research International pp. 1 - 11 |
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| Autores principales: | , , , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
12/19/2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=140476995&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 140476995 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 12/19/2019 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 140476995 140476995 140476995 10.1155/2019/6154867 140476995 ppf: 1 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH against Helicobacter pylori. aug: au: Lucio-Sauceda, Daniela Guadalupe Urrutia-Baca, Víctor Hugo Gomez-Flores, Ricardo De La Garza-Ramos, Myriam Angélica Tamez-Guerra, Patricia Orozco-Flores, Alonso affil: Laboratory of Immunology and Virology, School of Biological Sciences, Autonomous University of Nuevo Leon, Pedro de Alba and Manuel L. Barragán St., Cd. Universitaria, San Nicolás de los Garza, N.L., Monterrey, 66450, Mexico sug: subj: Mouthwashes Pharmacodynamics Microbiologic Phenomena Drug Effects Biofilms Drug Effects Hydrogen-Ion Concentration Helicobacter Pylori Drug Effects Helicobacter Infections Prevention and Control Mouth Diseases Prevention and Control Human Cytotoxicity Tests, Immunologic Gentian Violet Staining and Labeling Methods Gene Expression Drug Effects Lyases Drug Effects Bacterial Proteins Drug Effects Lipoproteins Drug Effects Acyltransferases Drug Effects Reverse Transcriptase Polymerase Chain Reaction Methods Gingiva Drug Effects Fibroblasts Drug Effects Cell Line Drug Effects Colorimetry Methods Biological Assay Methods ab: The presence of Helicobacter pylori in the oral cavity has been associated to the failure of antimicrobial therapy in patients with gastrointestinal infection and the development of oral diseases. However, it has been reported that the maintenance of good oral hygiene can improve the therapeutic success rates, where the use of mouthwashes with anti-Helicobacter activity would help to achieve it. The aim was to evaluate the antimicrobial activity of OxOral® mouthwash against H. pylori and its effect on biofilm formation. The minimum inhibitory concentration (MIC) of OxOral® (pH = 6.4–7.5, ORP = 650–900 mV) against H. pylori was calculated testing serial dilutions 0.117–15 ppm against 1 × 108 CFU/mL of H. pylori (ATCC® 700824™) by broth microdilution method using 96‐well plates. The H. pylori biofilm formation was determined by the optical density measurement at 600 nm from coverslips stained with 0.1% crystal violet. The gene expression of ureA, luxS, flaA, omp18, and lpxD were analyzed by RT‐qPCR. OxOral® cytotoxicity was evaluated in a human gingival fibroblast cell line by MTT assay. MIC was of 3.75 ppm, with 99.7 ± 7.7% bacterial growth inhibition. In the negative control, the biofilm formation was observed, whereas when bacteria were treated with OxOral® at 0.234, 0.469, and 0.938 ppm, an inhibition of 35.5 ± 0.9%, 89.1 ± 1.2%, and 99.9 ± 5.5% were obtained, respectively. The gene expression analysis showed that flaA, omp18, and lpxD genes were down‐regulated with OxOral® compared with control (p < 0.05). Low cytotoxicity of 16.5 ± 7.6% was observed at the highest dose (15 ppm); no significant differences were observed from 15 to 0.469 ppm compared to the control of untreated cells (p > 0.05). Our results reveal an important anti-Helicobacter activity of OxOral® and open the possibility of its therapeutic use new studies, which would increase the success rate of conventional therapies against H. pylori. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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