Excessive BCAA regulates fat metabolism partially through the modification of m6A RNA methylation in weanling piglets.
Background: Fat percentage and distribution in pigs are associated with their productive efficiency and meat quality. Dietary branched-chain amino acids (BCAA) regulate fat metabolism in weanling piglets with unknown mechanism. It is reported that N6-methyl-adenosine (m6A) is involved in fat metabol...
| Publicado en: | Nutrition & Metabolism Vol. 17; no. 1; pp. 1 - 13 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
BioMed Central
1/23/2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=141384208&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 141384208 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17437075 1CYX jtl: Nutrition & Metabolism issn: 17437075 maglogo: N pubinfo: dt: 1/23/2020 vid: 17 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 141384208 141384208 141384208 10.1186/s12986-019-0424-x 141384208 ppf: 1 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Excessive BCAA regulates fat metabolism partially through the modification of m6A RNA methylation in weanling piglets. aug: au: Heng, Jinghui Wu, Zhihui Tian, Min Chen, Jiaming Song, Hanqing Chen, Fang Guan, Wutai Zhang, Shihai affil: Guangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Wushan Avenue, Tianhe District, 510642, Guangzhou, China sug: subj: Diet Amino Acids, Branched-Chain Pharmacodynamics RNA, Messenger Drug Effects RNA Methylation Drug Effects Lipids Metabolism Adipose Tissue Drug Effects Lipids Drug Effects Liver Drug Effects Animal Studies Swine Amino Acids, Branched-Chain Administration and Dosage Leptin Drug Effects Adiponectin Drug Effects Fatty Acids Metabolism Gene Expression Drug Effects Peroxisome Proliferator-Activated Receptors Metabolism Restricted Diet Transcription Factors Drug Effects Spinal Nerve Roots Drug Effects Adipose Tissue Metabolism Liver Metabolism ab: Background: Fat percentage and distribution in pigs are associated with their productive efficiency and meat quality. Dietary branched-chain amino acids (BCAA) regulate fat metabolism in weanling piglets with unknown mechanism. It is reported that N6-methyl-adenosine (m6A) is involved in fat metabolism in mice. The current study was designed to investigate the relationship between dietary branched-chain amino acids and fat metabolism through N6-methyl-adenosine (m6A) in weanling piglets. Methods: A total of 18 healthy crossbred weaned piglets (Duroc × Landrace × Large White, 10.45 ± 0.41 kg) were divided into 3 treatments and were fed the low BCAA dose diet (L-BCAA), the normal dose BCAA diet (N-BCAA), or the high dose BCAA (H-BCAA) diet for 3 weeks. Results: Our results show that compared with the N-BCAA group, the L-BCAA group had higher concentration of serum leptin (P < 0.05), while the H-BCAA group had lower concentration of serum adiponectin (P < 0.05). Fatty acid synthesis in pigs from the H-BCAA group was lower than those from the N-BCAA group with the down-regulation of lipogenic genes (ACACA, FASN, PPAR-r, SREBP-1c in ventral and dorsal fat, SREBP-1c in liver) and up-regulation of lipolysis genes (HSL, ATGL, CPT-1A, FABP4 in ventral fat, HSL in liver) (P < 0.05). Similarly, fatty acid synthesis in pigs from the L-BCAA group was also lower than those from the N-BCAA group with the decrease of lipogenic genes (ACACA in ventral, ACACA and FASN in dorsal fat, ACACA, FASN, SREBP-1c in liver) and the increase of lipolysis genes (ATGL, CPT-1A CD36, FABP4 in ventral fat and HSL, ATGL, CPT-1A in dorsal fat, CPT-1A) (P < 0.05). Feeding H-BCAA diet significantly reduced total m6A levels in ventral and dorsal fat and liver tissues (P < 0.05). The decrease of total m6A is associated with down-regulation of METTL3, METTL14 and FTO in dorsal fat and METTL3 and FTO in liver (P < 0.05). Decreased m6A modification of ACACA and FASN in ventral and dorsal adipose tissues was observed in pig fed with excessive BCAA. Conclusion: These results suggest that insufficient or excessive BCAA decreased the fat deposition by increasing lipolysis and deceasing lipogenesis in adipose and liver tissues. Dietary excessive BCAA might regulate the process of lipid metabolism partly through the m6A RNA methylation. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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