Genome Survey and Transcriptome Analysis on Mycelia and Primordia of Agaricus blazei.

Agaricus blazei, a type of edible straw-rotting mushroom with somewhat sweet taste and fragrance of almonds, has attracted considerable scientific and practical attention. High-throughput Illumina PE150 and PacBio RSII platform were employed to generate a genomic sequence. De novo assembly generated...

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Publicado en:BioMed Research International pp. 1 - 14
Autores principales: Lu, Yuan-Ping, Liao, Jian-Hua, Guo, Zhong-Jie, Cai, Zhi-Xin, Chen, Mei-Yuan
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 1/14/2020
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 1/14/2020
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      pub: Wiley-Blackwell
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        10.1155/2020/1824183
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        atl: Genome Survey and Transcriptome Analysis on Mycelia and Primordia of Agaricus blazei.
      aug:
        au:
          Lu, Yuan-Ping
          Liao, Jian-Hua
          Guo, Zhong-Jie
          Cai, Zhi-Xin
          Chen, Mei-Yuan
        affil: Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, Fujian Province, China
      sug:
        subj:
          Mushroom, Edible Analysis
          Genome Evaluation
          Gene Expression Profiling Evaluation
          Sequence Analysis
          Arginine Analysis
          Steroids Analysis
          Biochemical Phenomena Evaluation
          Polysaccharides Analysis
          Aldehydes Analysis
      ab: Agaricus blazei, a type of edible straw-rotting mushroom with somewhat sweet taste and fragrance of almonds, has attracted considerable scientific and practical attention. High-throughput Illumina PE150 and PacBio RSII platform were employed to generate a genomic sequence. De novo assembly generated 36 contigs with 38,686,133 bp in size, containing 10,119 putative predicted genes. Additionally, we also studied transcriptional regulation of the mycelia and the primordia for exploration of genes involved in fruiting body formation. Expression profiling analysis revealed that 2,164 genes were upregulated in mycelia and 1,557 in primordia. Functional enrichment showed that differentially expressed genes associated with response to stress, ribosome biogenesis, arginine biosynthesis, and steroid biosynthesis pathway were more active in fruiting body. The genome and transcriptome analysis of A. blazei provide valuable sequence resources and contribute to our understanding of genes related to the biosynthesis pathway of polysaccharide and benzaldehyde, as well as the fruiting body formation.
      pubtype: Academic Journal
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        research
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      ougenre: Article
    language: English
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