Alterations in T cell-related transcripts at the feto-maternal interface throughout equine gestation.
Introduction: The tolerance of pregnancy by the maternal immune system is balanced between recognition and protection. In the human this is controlled by balancing helper T cell populations (Th1, Th2) in addition to immune suppression from the regulatory arm (Tregs), but this has not been evaluated...
| Published in: | Placenta Vol. 89; pp. 78 - 88 |
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| Main Authors: | , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Elsevier B.V.
Jan2020
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=141415570&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 141415570 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01434004 NZZ jtl: Placenta issn: 01434004 maglogo: N pubinfo: dt: Jan2020 vid: 89 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 141415570 141415570 NLM31730925 141415570 10.1016/j.placenta.2019.10.011 NLM31730925 141415570 ppf: 78 ppct: 10 formats: tig: atl: Alterations in T cell-related transcripts at the feto-maternal interface throughout equine gestation. aug: au: Fedorka, C.E. Loux, S.L. Scoggin, K.E. Adams, A.A. Troedsson, M.H.T. Ball, B.A. affil: Department of Veterinary Science, University of Kentucky, Lexington, KY, USA sug: subj: Placenta Metabolism Endometrium Metabolism RNA T Lymphocytes Metabolism Pregnancy Female Animal Studies Horses Genes Validation Studies Comparative Studies Evaluation Research Multicenter Studies Female ab: Introduction: The tolerance of pregnancy by the maternal immune system is balanced between recognition and protection. In the human this is controlled by balancing helper T cell populations (Th1, Th2) in addition to immune suppression from the regulatory arm (Tregs), but this has not been evaluated in the horse.Methods: RNA sequencing was performed on chorioallantois and endometrium of mares at 120, 180, 300 and 330 days of gestation (n = 4/stage), as well as 45-day chorioallantois (n = 4) and diestrus endometrium (n = 3). Transcripts were selected for relativity to Th1, Th2, or Treg-associated. qPCR and immunohistochemistry were used to confirm the results of select differentially expressed genes.Results: In the endometrium, Th1 transcripts were highest in the diestrus mare and decreased as gestational length progressed. In contrast, Th2 transcripts were upregulated in comparison to the diestrus mare and highest in mid gestation. Treg transcripts were found increased in comparison to the diestrus mare, but decreased prepartum. In the chorioallantois no Th1 transcripts changed. The majority of Th2 transcripts increased from 45 to 300 days gestation, and then decreased prepartum. Treg-related transcripts trended down in the chorioallantois from 45 days to 120 days gestation, followed by an upregulation to 300 days and a secondary decline prepartum.Discussion: The mare experiences a complex and evolving immune profile within the tissues of the feto-maternal interface. This consists of a balance between the Th1 and Th2 response, and a dynamic Treg response that is hypothesized to regulate overall events within the immune system. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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