ESR1 mutations are frequent in newly diagnosed metastatic and loco-regional recurrence of endocrine-treated breast cancer and carry worse prognosis.

Background: Emerging mutations in the ESR1 gene that encodes for the estrogen receptor (ER) are associated with resistance to endocrine therapy. ESR1 mutations rarely exist in primary tumors (~ 1%) but are relatively common (10-50%) in metastatic, endocrine therapy-resistant cancers and are associat...

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Publicado en:Breast Cancer Research Vol. 22; no. 1; pp. 1 - 12
Autores principales: Zundelevich, Adi, Dadiani, Maya, Kahana-Edwin, Smadar, Itay, Amit, Sella, Tal, Gadot, Moran, Cesarkas, Karen, Farage-Barhom, Sarit, Saar, Efrat Glick, Eyal, Eran, Kol, Nitzan, Pavlovski, Anya, Balint-Lahat, Nora, Dick-Necula, Daniela, Barshack, Iris, Kaufman, Bella, Gal-Yam, Einav Nili
Formato: research Journal Article
Publicado: BioMed Central 2/3/2020
Acceso en línea:Ver este registro en EBSCOhost
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        14655411
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      dt: 2/3/2020
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      pub: BioMed Central
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        141530857
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        141530857
        10.1186/s13058-020-1246-5
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        atl: ESR1 mutations are frequent in newly diagnosed metastatic and loco-regional recurrence of endocrine-treated breast cancer and carry worse prognosis.
      aug:
        au:
          Zundelevich, Adi
          Dadiani, Maya
          Kahana-Edwin, Smadar
          Itay, Amit
          Sella, Tal
          Gadot, Moran
          Cesarkas, Karen
          Farage-Barhom, Sarit
          Saar, Efrat Glick
          Eyal, Eran
          Kol, Nitzan
          Pavlovski, Anya
          Balint-Lahat, Nora
          Dick-Necula, Daniela
          Barshack, Iris
          Kaufman, Bella
          Gal-Yam, Einav Nili
        affil: Cancer Research Center, Sheba Medical Center, Tel-Hashomer, Israel
      sug:
        subj:
          Antineoplastic Agents, Hormonal Pharmacodynamics
          Breast Neoplasms Mortality
          Neoplasms, Hormone-Dependent Mortality
          Drug Resistance, Neoplasm
          Proteins
          Mutation
          Neoplasm Recurrence, Local Mortality
          Aged
          Adult
          Treatment Outcomes
          Breast Neoplasms Pathology
          Middle Age
          Breast Neoplasms Drug Therapy
          Neoplasms, Hormone-Dependent Pathology
          Aged, 80 and Over
          Neoplasms, Hormone-Dependent Drug Therapy
          Neoplasm Recurrence, Local Pathology
          Neoplasm Recurrence, Local
          Breast Neoplasms
          Neoplasm Metastasis
          Female
          Neoplasm Recurrence, Local Drug Therapy
          Neoplasms, Hormone-Dependent
          Human
          Survival
          Validation Studies
          Comparative Studies
          Evaluation Research
          Multicenter Studies
          Scales
          Aged: 65+ years
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Aged, 80 & over
          Female
      ab: Background: Emerging mutations in the ESR1 gene that encodes for the estrogen receptor (ER) are associated with resistance to endocrine therapy. ESR1 mutations rarely exist in primary tumors (~ 1%) but are relatively common (10-50%) in metastatic, endocrine therapy-resistant cancers and are associated with a shorter progression-free survival. Little is known about the incidence and clinical implication of these mutations in early recurrence events, such as local recurrences or newly diagnosed metastatic disease.Methods: We collected 130 archival tumor samples from 103 breast cancer patients treated with endocrine therapy prior to their local/metastatic recurrence. The cohort consisted of 41 patients having at least 1 sample from local/loco-regional recurrence and 62 patients with metastatic disease (of whom 41 newly diagnosed and 28 with advanced disease). The 5 most common ESR1 hotspot mutations (D538G, L536R, Y537S/N/C) were analyzed either by targeted sequencing or by droplet digital PCR. Progression-free survival (PFS), disease-free survival (DFS), and distant recurrence-free survival (DRFS) were statistically tested by Kaplan-Meier analysis.Results: The prevalence of ESR1 mutations was 5/41 (12%) in newly diagnosed metastatic patients and 5/28 (18%) for advanced metastases, detected at allele frequency > 1%. All mutations in advanced metastases were detected in patients previously treated with both tamoxifen (TAM) and aromatase inhibitors (AI). However, in newly diagnosed metastatic patients, 4/5 mutations occurred in patients treated with TAM alone. PFS on AI treatment in metastatic patients was significantly shorter for ESR1 mutation carriers (p = 0.017). In the local recurrence cohort, ESR1 mutations were identified in 15/41 (36%) patients but only 4/41 (10%) were detected at allele frequency > 1%. Again, most mutations (3/4) were detected under TAM monotherapy. Notably, 1 patient developed ESR1 mutation while on neoadjuvant endocrine therapy. DFS and DRFS were significantly shorter (p = 0.04 and p = 0.017, respectively) in patients that had ESR1 mutations (> 1%) in their loco-regional recurrence tumor.Conclusions: Clinically relevant ESR1 mutations are prevalent in newly diagnosed metastatic and local recurrence of endocrine-treated breast cancer. Since local recurrences are amenable to curative therapy, these mutations may inform the selection of subsequent endocrine therapies.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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