Assessment of changes in growth traits, oxidative stress parameters, and enzymatic and non-enzymatic antioxidant defense mechanisms in Lepidium draba plant under osmotic stress induced by polyethylene glycol.
Lepidium draba is a weed with the medicinal properties which few researches have been done on it. In this study, some traits, related to the osmotic stress, in 14-day-old L. draba sprouts that were grown 9 days in the presence of various doses of polyethylene glycol 6000 (PEG 6000) including 0, 3, 6...
| Publicado en: | Protoplasma Vol. 257; no. 2; pp. 459 - 474 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Mar2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=141898143&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 141898143 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: Mar2020 vid: 257 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 141898143 10.1007/s00709-019-01457-0 141898143 ppf: 459 ppct: 15 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Assessment of changes in growth traits, oxidative stress parameters, and enzymatic and non-enzymatic antioxidant defense mechanisms in Lepidium draba plant under osmotic stress induced by polyethylene glycol. aug: au: Jamshidi Goharrizi, Kiarash Moosavi, Sayyed Saeed Amirmahani, Farzane Salehi, Fatemeh Nazari, Maryam affil: Department of Plant Breeding, Yazd Branch, Islamic Azad University, Yazd, Iran sug: ab: Lepidium draba is a weed with the medicinal properties which few researches have been done on it. In this study, some traits, related to the osmotic stress, in 14-day-old L. draba sprouts that were grown 9 days in the presence of various doses of polyethylene glycol 6000 (PEG 6000) including 0, 3, 6, 9, and 12%, with different osmotic potentials (− 0.04, − 0.12, − 0.23, − 0.34, and − 0.48 MPa, respectively) were investigated. Based on our results, germination percentage besides stem and root lengths decreased with increasing the concentrations of PEG. The contents of electrolyte leakage, malondialdehyde, other aldehydes, total protein, free amino acids, total soluble carbohydrate as well as free proline increased with increasing the concentrations of PEG. Also, for the first time, our results have proven that under osmotic stress, there is an adverse relationship between hydrogen peroxide content and the activity of catalase, peroxidase, ascorbate peroxidase, and guaiacol peroxidase enzymes, such that hydrogen peroxide content decreased with induction of PEG up to 6% and after that increased, while the activity of catalase, peroxidase, ascorbate peroxidase, and guaiacol peroxidase enzymes increased up to 6% PEG and after that decreased. The expression levels of catalase, peroxidase, ascorbate peroxidase, and guaiacol peroxidase genes showed the same pattern as was seen for these enzyme activities. According to the results of this study, it can be deduced that decreasing H2O2 content cannot be the main reason for other oxidative stress parameters to decrease. In this study, P5CS and P5CR gene expression levels increased with increasing levels of PEG up to 12% which was completely similar to free proline content. Based on our results, L. draba can be considered as a semi-tolerant plant to osmotic stress. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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