CD147 Expression Is Associated with Tumor Proliferation in Bladder Cancer via GSDMD.

Purpose. CD147, also known as BSG, is a type I transmembrane glycoprotein that belonged to immunoglobulin superfamily. Mature CD147 is an N-linked glycosylated protein and exists on the transmembrane and as soluble forms in tumors. However, the function of CD147 in cell proliferation of bladder canc...

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Publicado en:BioMed Research International pp. 1 - 8
Autores principales: Peng, Junming, Jiang, Hongtao, Guo, Jinan, Huang, Jiansheng, Yuan, Qian, Xie, Jing, Xiao, Kefeng
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 2/21/2020
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: BioMed Research International
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      dt: 2/21/2020
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        141922473
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        10.1155/2020/7638975
        141922473
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        atl: CD147 Expression Is Associated with Tumor Proliferation in Bladder Cancer via GSDMD.
      aug:
        au:
          Peng, Junming
          Jiang, Hongtao
          Guo, Jinan
          Huang, Jiansheng
          Yuan, Qian
          Xie, Jing
          Xiao, Kefeng
        affil: Department of Urology, The First Affiliated Hospital of Southern University of Science and Technology/Shenzhen People's Hospital, Shenzhen, Guangdong 518000, China
      sug:
        subj:
          Membrane Glycoproteins Metabolism
          Gene Expression
          Cell Line, Tumor Metabolism
          Cell Proliferation
          Intracellular Signaling Peptides and Proteins Metabolism
          Bladder Neoplasms Prognosis
          Human
          Immunohistochemistry
          Blotting, Western
          Tumor Markers, Biological Metabolism
          Survival
      ab: Purpose. CD147, also known as BSG, is a type I transmembrane glycoprotein that belonged to immunoglobulin superfamily. Mature CD147 is an N-linked glycosylated protein and exists on the transmembrane and as soluble forms in tumors. However, the function of CD147 in cell proliferation of bladder cancer (BC) remains to be elucidated. Methods. The study included 159 patients with BC and 68 healthy controls. The expression of CD147 and gasdermin D (GSDMD) was analyzed by immunohistochemistry (IHC). Western blotting was performed to detect the expression of proteins in BC cells. The relationship between CD147 and GSDMD was analyzed by the IHC score. Results. The expression of CD147 was significantly increased in BC when compared to healthy controls, and the level of CD147 was correlated with tumor proliferation characterized by Ki-67, which is a cell proliferation antigen. In addition, CD147 treatment of BC cells increased the expression of GSDMD, leading to increased Ki-67 expression, while CD147 blockade with peptide in BC significantly reduced GSDMD expression, resulting in reduced cell proliferation. Furthermore, overexpression of GSDMD markedly overcame the inhibitory effect of CD147 peptide on tumor proliferation. BC patients with overexpression of CD147 showed correlation with GSDMD and demonstrated significantly poorer prognosis and overall survival rate. Conclusion. These findings suggested that high expression of CD147 contributed to tumor proliferation in BC via GSDMD, which might in turn act as an unfavorable prognostic marker.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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