Novel Antimicrobial Peptides Formulated in Chitosan Matrices are Effective Against Biofilms of Multidrug-Resistant Wound Pathogens.
Introduction: Infection frequently complicates the treatment of combat-related wounds, impairs healing, and leads to worse outcomes. To better manage wound infections, antimicrobial therapies that are effective against biofilm and designed for direct wound application are needed. The primary objecti...
| Publicado en: | Military Medicine Vol. 185; pp. 637 - 644 |
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| Autores principales: | , , , |
| Formato: | Journal Article |
| Publicado: |
Oxford University Press / USA
2020 Supplement
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=141923057&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 141923057 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00264075 4DV jtl: Military Medicine issn: 00264075 maglogo: N pubinfo: dt: 2020 Supplement vid: 185 pid: 622 pub: Oxford University Press / USA artinfo: ui: 141923057 141923057 NLM32074338 10.1093/milmed/usz222 NLM32074338 141923057 ppf: 637 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Novel Antimicrobial Peptides Formulated in Chitosan Matrices are Effective Against Biofilms of Multidrug-Resistant Wound Pathogens. aug: au: Neff, Jennifer A Bayramov, Danir F Patel, Esha A Miao, Jing affil: Allvivo Vascular, Inc., 20914 Bake Parkway, Suite 100, Lake Forest, CA 92630 sug: subj: Biofilms Drug Effects Polysaccharides Pharmacokinetics Antimicrobial Peptides Bandages and Dressings Statistics and Numerical Data Swine Proteins Pharmacokinetics Proteins Therapeutic Use Wound Healing Drug Effects Drug Resistance Gels Therapeutic Use Bandages and Dressings Standards Polysaccharides Therapeutic Use Animals Models, Biological Funding Source ab: Introduction: Infection frequently complicates the treatment of combat-related wounds, impairs healing, and leads to worse outcomes. To better manage wound infections, antimicrobial therapies that are effective against biofilm and designed for direct wound application are needed. The primary objective of this work was to evaluate a chitosan matrix for delivery of two engineered antimicrobial peptides, (ASP)-1 and ASP-2, to treat biofilm-associated bacteria. A secondary objective was to determine whether replacing the levorotatory (L) form amino acids in ASP-2 with dextrorotatory (D) form amino acids would impact peptide activity.Materials and Methods: Chitosan gels loaded with antimicrobial peptides were evaluated for peptide release over 7 days and tested for efficacy against biofilms grown both in vitro on polymer mesh and ex vivo on porcine skin.Results: When delivered via chitosan, 70% to 80% of peptides were released over 7 days. Gels eradicated biofilms of gram-positive and gram-negative, drug-resistant bacteria in vitro and ex vivo. Under the conditions tested, no meaningful differences in peptide activity between the L and D forms of ASP-2 were detected.Conclusions: Chitosan serves as an effective delivery platform for ASP-1 and ASP-2 to treat biofilm-embedded bacteria and warrants further development as a topical treatment. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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