A novel random forest integrative approach based on endogenous CYP3A4 phenotype for predicting tacrolimus concentrations and dosages in Chinese renal transplant patients.

What is known and objective: Personalized treatment with tacrolimus has remained a challenge. The present study aimed to evaluate the potential of an integrative approach to predict individual tacrolimus concentrations and dosages based on endogenous CYP3A4 phenotype, CYP3A5 genotype and clinical va...

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Published in:Journal of Clinical Pharmacy & Therapeutics Vol. 45; no. 2; pp. 318 - 324
Main Authors: Cai, Ningfang, Zhang, Xiujin, Zheng, Chao, Zhu, Lijun, Zhu, Minfeng, Cheng, Zeneng, Luo, Xi
Format: research tables/charts Journal Article
Published: Wiley-Blackwell Apr2020
Online Access:View this record in EBSCOhost
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      dt: Apr2020
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1111/jcpt.13074
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        atl: A novel random forest integrative approach based on endogenous CYP3A4 phenotype for predicting tacrolimus concentrations and dosages in Chinese renal transplant patients.
      aug:
        au:
          Cai, Ningfang
          Zhang, Xiujin
          Zheng, Chao
          Zhu, Lijun
          Zhu, Minfeng
          Cheng, Zeneng
          Luo, Xi
        affil: Department of Pharmacy, Xiamen Children's Hospital, Xiamen, China
      sug:
        subj:
          Cytochrome P-450 Enzyme System Urine
          Phenotype Evaluation
          Genotype Evaluation
          Biological Markers China
          Tacrolimus Pharmacokinetics
          Tacrolimus Administration and Dosage
          Kidney Transplantation China
          Transplant Recipients China
          Human
          Chinese Persons
          China
          Algorithms
          Urinalysis
          Hydrocortisone Urine
          Cortisone Urine
      ab: What is known and objective: Personalized treatment with tacrolimus has remained a challenge. The present study aimed to evaluate the potential of an integrative approach to predict individual tacrolimus concentrations and dosages based on endogenous CYP3A4 phenotype, CYP3A5 genotype and clinical variables. Methods: A random forest (RF) algorithm which incorporated an endogenous CYP3A4 phenotype (assessed by urinary ratio of 6β‐hydroxycortisol and 6β‐hydroxycortisone to cortisol and cortisone), CYP3A5*3 genotype and other clinical determinants of tacrolimus disposition was performed in 182 medically stable renal transplant recipients. Results and discussion: The results suggested that endogenous CYP3A4 phenotype was the most important determinant of tacrolimus concentrations and dose requirements. RF models provided high goodness of fit (R2) with.92 and.95 for the prediction of tacrolimus trough concentrations and dosages, respectively, as well as high predictability (Q2) with 0.63 and 0.70, respectively. Significant correlations existed between experimental and predictive data. What is new and conclusion: In summary, endogenous CYP3A4 phenotype is a critical biomarker for the determination of tacrolimus disposition. This predictive RF approach based on CYP3A4 biomarker with the combination of CYP3A5*3 genotype and other clinical variables can be used for predicting tacrolimus concentrations and dosages, which may serve as a useful tool in individualized tacrolimus dosing.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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