Short‐wave infrared light imaging measures tissue moisture and distinguishes superficial from deep burns.

Existing clinical approaches and tools to measure burn tissue destruction are limited resulting in misdiagnosis of injury depth in over 40% of cases. Thus, our objective in this study was to characterize the ability of short‐wave infrared (SWIR) imaging to detect moisture levels as a surrogate for t...

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Publicado en:Wound Repair & Regeneration Vol. 28; no. 2; pp. 185 - 194
Autores principales: Mironov, Sergey, Hwang, Charles D., Nemzek, Jean, Li, John, Ranganathan, Kavitha, Butts, Jonathan T., Cholok, David J., Dolgachev, Vladislav A., Wang, Stewart C., Hemmila, Mark, Cederna, Paul S., Morris, Michael D., Berenfeld, Omer, Levi, Benjamin
Formato: diagnostic images pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell Mar2020
Acceso en línea:Ver este registro en EBSCOhost
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        atl: Short‐wave infrared light imaging measures tissue moisture and distinguishes superficial from deep burns.
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        au:
          Mironov, Sergey
          Hwang, Charles D.
          Nemzek, Jean
          Li, John
          Ranganathan, Kavitha
          Butts, Jonathan T.
          Cholok, David J.
          Dolgachev, Vladislav A.
          Wang, Stewart C.
          Hemmila, Mark
          Cederna, Paul S.
          Morris, Michael D.
          Berenfeld, Omer
          Levi, Benjamin
        affil: Center for Arrhythmia Research, University of Michigan, Ann Arbor Michigan
      sug:
        subj:
          Tissue Analysis
          Body Water Analysis
          Biological Phenomena
          Spectrophotometry, Infrared Methods
          Diagnostic Imaging Methods
          Burns Diagnosis
          Validity Evaluation
          Animal Studies
          Descriptive Statistics
          Biopsy
          In Vivo Studies
          Mice
          Swine
          Validation Studies
          Histology
      ab: Existing clinical approaches and tools to measure burn tissue destruction are limited resulting in misdiagnosis of injury depth in over 40% of cases. Thus, our objective in this study was to characterize the ability of short‐wave infrared (SWIR) imaging to detect moisture levels as a surrogate for tissue viability with resolution to differentiate between burns of various depths. To accomplish our aim, we constructed an imaging system consisting of a broad‐band Tungsten light source; 1,200‐, 1,650‐, 1,940‐, and 2,250‐nm wavelength filters; and a specialized SWIR camera. We initially used agar slabs to provide a baseline spectrum for SWIR light imaging and demonstrated the differential absorbance at the multiple wavelengths, with 1,940 nm being the highest absorbed wavelength. These spectral bands were then demonstrated to detect levels of moisture in inorganic and in vivo mice models. The multiwavelength SWIR imaging approach was used to diagnose depth of burns using an in vivo porcine burn model. Healthy and injured skin regions were imaged 72 hours after short (20 seconds) and long (60 seconds) burn application, and biopsies were extracted from those regions for histologic analysis. Burn depth analysis based on collagen coagulation histology confirmed the formation of superficial and deep burns. SWIR multispectral reflectance imaging showed enhanced intensity levels in long burned regions, which correlated with histology and distinguished between superficial and deep burns. This SWIR imaging method represents a novel, real‐time method to objectively distinguishing superficial from deep burns.
      pubtype: Academic Journal
      doctype:
        diagnostic images
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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