Effects of Four Kinds of Oxide Nanoparticles on Proteins in Extracellular Polymeric Substances of Sludge.
Proteins are the most important component in sludge extracellular polymeric substances (EPS) and play a crucial role in the formation of sludge flocs, adsorption performance of sludge, and flocculation ability of sludge. This research is aimed at exploring the changes in proteins in EPS extracted fr...
| Publicado en: | BioMed Research International pp. 1 - 14 |
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| Autores principales: | , , , , |
| Formato: | protocol research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
3/3/2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=142252576&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 142252576 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 3/3/2020 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 142252576 142252576 142252576 10.1155/2020/1754134 142252576 ppf: 1 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Effects of Four Kinds of Oxide Nanoparticles on Proteins in Extracellular Polymeric Substances of Sludge. aug: au: Wang, Weiyun Zang, Ying Wang, Cijia Wang, Kangni Li, Rundong affil: College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, China sug: subj: Nanoparticles Oxides Analysis Extracellular Space Analysis Biofilms Analysis Peptides Analysis Human Aluminum Oxide Analysis Ferric Compounds Analysis Zinc Oxide Analysis Titanium Analysis Spectrometry, Fluorescence Methods Imaging, Three-Dimensional Methods Tyrosine Analysis Amides Analysis ab: Proteins are the most important component in sludge extracellular polymeric substances (EPS) and play a crucial role in the formation of sludge flocs, adsorption performance of sludge, and flocculation ability of sludge. This research is aimed at exploring the changes in proteins in EPS extracted from concentrated sludge after various nanoparticle (NP) treatments. The results showed that the protein content in EPS decreased by 40% after nanoalumina (Al2O3 NPs) treatment but increased at varying degrees after nanoferric oxide (Fe3O4 NPs), nanozinc oxide (ZnO NPs), and nanotitanium dioxide (TiO2 NPs) treatments. The four kinds of nanoparticles not only affected the protein content in EPS but also influenced the types and structures of proteins. The results of three-dimensional fluorescence spectroscopy showed that the tyrosine-like protein content in soluble EPS (SEPS) decreased after treatments with four kinds of NPs. Infrared spectroscopy analysis revealed that the absorption intensity of amide I and amide II weakened after Al2O3 NP treatment, whereas that of amide I enhanced after Fe3O4 NP, ZnO NP, and TiO2 NP treatments. Further analysis of the secondary structure of proteins in the infrared range of 1700–1600 cm-1 demonstrated that the value of α-helix/(β-sheet+random coil) decreased from 0.513 to 0.383 in SEPS after TiO2 NP treatment. For the samples treated by Fe3O4 NPs, the percentage of α-helix significantly increased and that of β-sheet slightly decreased in proteins from SEPS and loosely bound EPS. pubtype: Academic Journal doctype: protocol research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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