PinX1t, a Novel PinX1 Transcript Variant, Positively Regulates Cardiogenesis of Embryonic Stem Cells.
Background Pin2/TRF1-interacting protein, PinX1, was previously identified as a tumor suppressor. Here, we discovered a novel transcript variant of mPinX1 (mouse PinX1), mPinX1t (mouse PinX1t), in embryonic stem cells (ESCs). The aims of this investigation were (1) to detect the presence of mPinX1 a...
| Publicado en: | Journal of the American Heart Association Vol. 9; no. 6; pp. 1 - 56 |
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| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
3/17/2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=142359285&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 142359285 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20479980 FSQ9 jtl: Journal of the American Heart Association issn: 20479980 maglogo: Y pubinfo: dt: 3/17/2020 vid: 9 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 142359285 142359285 NLM32157956 142359285 10.1161/JAHA.118.010240 NLM32157956 142359285 ppf: 1 ppct: 55 formats: tig: atl: PinX1t, a Novel PinX1 Transcript Variant, Positively Regulates Cardiogenesis of Embryonic Stem Cells. aug: au: Hing Chung Chan Yuen Ting Lau Qianqian Ding Chun Kit Li Chi Ming Wong Pang Chui Shaw Mary Miu Yee Waye Suk Ying Tsang Chan, Hing Chung Lau, Yuen Ting Ding, Qianqian Li, Chun Kit Wong, Chi Ming Shaw, Pang Chui Waye, Mary Miu Yee Tsang, Suk Ying affil: School of Life Sciences, Hong Kong SAR sug: subj: Cell Differentiation Proteins Metabolism Cell Cycle Proteins Metabolism Cells Metabolism Carrier Proteins Morphogenesis Carrier Proteins Metabolism Transcription Factors Animal Studies Genes Histocompatibility Antigens RNA Metabolism Transcription Factors Metabolism RNA Cell Line Proteins Signal Transduction Mice Histocompatibility Antigens Metabolism Cell Cycle Proteins Comparative Studies Multicenter Studies Evaluation Research Validation Studies ab: Background Pin2/TRF1-interacting protein, PinX1, was previously identified as a tumor suppressor. Here, we discovered a novel transcript variant of mPinX1 (mouse PinX1), mPinX1t (mouse PinX1t), in embryonic stem cells (ESCs). The aims of this investigation were (1) to detect the presence of mPinX1 and mPinX1t in ESCs and their differentiation derivatives; (2) to investigate the role of mPinX1 and mPinX1t on regulating the characteristics of undifferentiated ESCs and the cardiac differentiation of ESCs; (3) to elucidate the molecular mechanisms of how mPinX1 and mPinX1t regulate the cardiac differentiation of ESCs. Methods and Results By 5' rapid amplification of cDNA ends, 3' rapid amplification of cDNA ends, and polysome fractionation followed by reverse transcription-polymerase chain reaction, mPinX1t transcript was confirmed to be an intact mRNA that is actively translated. Western blot confirmed the existence of mPinX1t protein. Overexpression or knockdown of mPinX1 (both decreased mPinX1t expression) both decreased while overexpression of mPinX1t increased the cardiac differentiation of ESCs. Although both mPinX1 and mPinX1t proteins were found to bind to cardiac transcription factor mRNAs, only mPinX1t protein but not mPinX1 protein was found to bind to nucleoporin 133 protein, a nuclear pore complex component. In addition, mPinX1t-containing cells were found to have a higher cytosol-to-nucleus ratio of cardiac transcription factor mRNAs when compared with that in the control cells. Our data suggested that mPinX1t may positively regulate cardiac differentiation by enhancing export of cardiac transcription factor mRNAs through interacting with nucleoporin 133. Conclusions We discovered a novel transcript variant of mPinX1, the mPinX1t, which positively regulates the cardiac differentiation of ESCs. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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