Protective effect of bacterial lipase on lipopolysaccharide-induced toxicity in rat cardiomyocytes; H9C2 cell line.
Introduction: Cardiovascular system is highly sensitive to LPS-induced oxidative damage. This study aimed to show the inhibitory effect of bacterial Lipase on LPS-induced cardiomyoblasts toxicity. Methods: Rat cardiomyoblasts H9C2 were classified into Control, LPS (cells received 0.1, 1 and 10 μg/mL...
| Publicado en: | Journal of Cardiovascular & Thoracic Research Vol. 12; no. 1; pp. 35 - 43 |
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| Autores principales: | , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Tabriz University of Medical Sciences
2020
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=142396252&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 142396252 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20085117 BL7R jtl: Journal of Cardiovascular & Thoracic Research issn: 20085117 maglogo: N pubinfo: dt: 2020 vid: 12 iid: 1 pid: 69573 pub: Tabriz University of Medical Sciences artinfo: ui: 142396252 142396252 142396252 10.34172/jcvtr.2020.06 142396252 ppf: 35 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Protective effect of bacterial lipase on lipopolysaccharide-induced toxicity in rat cardiomyocytes; H9C2 cell line. aug: au: Mamipour, Mina Yousefi, Mohammadreza Dehnad, Alireza Faridvand, Yousef Zarezadeh, Reza Khaksar, Majid Pouyafar, Ayda Rahbarghazi, Reza affil: Department of Biotechnology, Higher Education Institute of Rab-Rashid, Tabriz, Iran sug: subj: Bacteria Lipase Pharmacodynamics Myocytes, Cardiac Drug Effects Cell Line Drug Effects Oxidative Stress Drug Effects Animal Studies Rats Cell Viability Colorimetry Gene Expression Signal Transduction Toll-Like Receptors Metabolism Polymerase Chain Reaction Fatty Acids Analysis Chromatography, Gas Nitric Oxide Analysis NF-kappa B Drug Effects ab: Introduction: Cardiovascular system is highly sensitive to LPS-induced oxidative damage. This study aimed to show the inhibitory effect of bacterial Lipase on LPS-induced cardiomyoblasts toxicity. Methods: Rat cardiomyoblasts H9C2 were classified into Control, LPS (cells received 0.1, 1 and 10 μg/mL LPS) and LPS+ Lipase groups. In LPS+Lipase group, different concentrations of lipopolysaccharide were pre-incubated with 5 mg/mL bacterial lipase at 37˚C overnight prior to cell treatment. After 72 hours, cell viability was assessed by MTT assay. The expression of key genes related to toll-like receptor signaling pathways was assessed by real-time PCR assay. Percentage of fatty acids was evaluated in each group using gas chromatography assay. The levels of NO was also measured using the Griess reaction. Results: Data showed H9C2 cells viability was decreased after exposure to LPS in a dose-dependent manner (P < 0.05). Incubation of LPS with lipase increased cell survival rate and closed to near-to-control levels (P < 0.05). Lipase had the potential to blunt the increased expression of IRAK and NF-κB in cells after exposure to the LPS. Compared to the LPS group, lipase attenuated the increased level of NO-induced by LPS (P < 0.05). Gas chromatography analysis showed the reduction of saturated fatty acids in cells from LPS group while the activity of lipase prohibited impact of LPS on cell fatty acid composition. LPS decreased the ability of cardiomyoblasts to form colonies. Incubation of LPS with lipase enhanced clonogenic capacity. Conclusion: Reduction in lipopolysaccharide-induced cytotoxicity is possibly related to lipase activity and reduction of modified lipopolysaccharide with toll-like receptor. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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