Intracranial Infections in Neurological Surgery: The Changes of Circular RNA Expression and Their Possible Function Mechanism.
Background and Objective. Circular RNA (circRNA) often has high stability and has been widely studied as diagnostic markers in many diseases, including neurodegenerative disorders, diabetes, and various types of cancers. In this study, circRNA was evaluated as a diagnostic marker for central nervous...
| Publicado en: | BioMed Research International pp. 1 - 9 |
|---|---|
| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
5/5/2020
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=143058104&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 143058104 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 5/5/2020 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 143058104 143058104 143058104 10.1155/2020/2536272 143058104 ppf: 1 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Intracranial Infections in Neurological Surgery: The Changes of Circular RNA Expression and Their Possible Function Mechanism. aug: au: Niu, Jiangtao Xu, Hongqi Yang, Bo affil: Neurosurgery Department, First Affiliated Hospital of Zhengzhou University, Henan Province, China sug: subj: RNA, Circular Cerebrospinal Fluid Biological Markers Cerebrospinal Fluid Gene Expression Central Nervous System Infections Diagnosis Brain Diseases Diagnosis Neurosurgery Adverse Effects Brain Diseases Etiology Central Nervous System Infections Etiology Human Polymerase Chain Reaction Methods Oligonucleotide Array Sequence Analysis Bioinformatics T-Tests Insulin Resistance Metabolism Signal Transduction Transcription Factors Metabolism Protein Kinases Metabolism Endometrial Neoplasms Metabolism Transforming Growth Factor beta Metabolism RNA, Circular Metabolism Biological Markers Metabolism ab: Background and Objective. Circular RNA (circRNA) often has high stability and has been widely studied as diagnostic markers in many diseases, including neurodegenerative disorders, diabetes, and various types of cancers. In this study, circRNA was evaluated as a diagnostic marker for central nervous system infection in neurological surgery. Methods. circRNA expression was analysed in six cerebrospinal fluid (CSF) samples from three patients of the infectious and noninfectious phases using an Arraystar Human circRNA Array. Differentially altered circRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR) in the 66 CSF samples of 33 patients of the infectious and noninfectious phases. t -test was used for statistical analysis. A bioinformatics analysis was employed to investigate the function mechanism of the circRNAs. Results. Firstly, 142 circRNAs were found significantly different in 6 CSF samples of the infection and noninfection phases of 3 patients. Fourteen circRNAs with the top largest fold changes were chosen from the 142 circRNAs for PCR validation in the same 6 CSF samples of 3 patients. Three circRNAs were selected to be validated in 60 CSF samples of 30 patients using the PCR test. In infection CSF, an upregulated hsa_circRNA_402632 and downregulated hsa_circRNA_008636 and hsa_circRNA_405481 were confirmed by PCR test. A bioinformatics analysis was used to investigate the function mechanism of the 3 circRNAs. hsa_circRNA_402632 is enriched in the insulin resistance pathway, the FoxO and AMPK signaling pathways are the most important pathways for hsa_circRNA_008636 gene expression, and hsa_circRNA_405481 is enriched in the endometrial cancer signaling pathway, Fc epsilon RI signaling pathway, and TGF-beta signaling pathway. Conclusions. hsa_circRNA_402632, hsa_circRNA_008636, and hsa_circRNA_405481 may be potential diagnostic markers for central nervous system infection after neurological surgery. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|