Transcript profiling of genes involved in carotenoid biosynthesis among three carrot cultivars with various taproot colors.
Carotenoids are a group of natural pigments that are widely distributed in various plants. Carrots are plants rich in carotenoids and have fleshy roots with different colors. Carotenoid accumulation is a complex regulatory process with important guiding significance for carrot production. In this wo...
| Published in: | Protoplasma Vol. 257; no. 3; pp. 949 - 964 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Published: |
Springer Nature
May2020
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=143073042&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 143073042 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: May2020 vid: 257 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 143073042 10.1007/s00709-020-01482-4 143073042 ppf: 949 ppct: 15 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Transcript profiling of genes involved in carotenoid biosynthesis among three carrot cultivars with various taproot colors. aug: au: Wang, Ya-Hui Li, Tong Zhang, Rong-Rong Khadr, Ahmed Tian, Yong-Sheng Xu, Zhi-Sheng Xiong, Ai-Sheng affil: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, 1 Weigang, 210095, Nanjing, China sug: ab: Carotenoids are a group of natural pigments that are widely distributed in various plants. Carrots are plants rich in carotenoids and have fleshy roots with different colors. Carotenoid accumulation is a complex regulatory process with important guiding significance for carrot production. In this work, three carrot cultivars with different taproot colors, Hongxinqicun (orange), Benhongjinshi (red), and Tianzi (purple) were chosen as experimental materials to explore the molecular mechanism of carotenoid accumulation in carrot. Results showed that the three carotenoids, namely, α-carotene, β-carotene, and lutein, had accumulated in orange carrot cultivar Hongxinqicun. Lycopene was only detected in the taproots of Benhongjinshi. Lutein was the main carotenoid in Tianzi. Comparison of the carotenoid contents in different tissues of carrot showed that leaf blade was the tissue with the highest carotenoid accumulation. Expression analysis of carotenoid biosynthesis genes and its correlation with carotenoid accumulation confirmed the regulatory role of structural genes in carrots. The high expression of five lycopene synthesis-related genes, DcPSY2, DcPDS, DcZDS1, DcCRT1, DcCRT2, and low expression of DcLCYE may result in the lycopene accumulation in Benhongjinshi. However, the function of certain genes, such as DcPSY1 that was lowly expressed in red carrot, requires further investigation. Our results provided potential insights into the mechanism of carotenoid accumulation in three carrot cultivars with different taproot colors. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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